Transvection mediated by the translocated cyclin D1 locus in mantle cell lymphoma.
Transvection mediated by the translocated cyclin D1 locus in mantle cell lymphoma.
复制标题
DOI：
10.1084/jem.20072102
复制
发表时间：
2008-08-04
期刊：
影响因子：
--
通讯作者：
Epner E
中科院分区：
文献类型：
--
作者：
Liu H;Huang J;Wang J;Jiang S;Bailey AS;Goldman DC;Welcker M;Bedell V;Slovak ML;Clurman B;Thayer M;Fleming WH;Epner E
In mantle cell lymphoma (MCL) and some cases of multiple myeloma (MM), cyclin D1 expression is deregulated by chromosome translocations involving the immunoglobulin heavy chain (IgH) locus. To evaluate the mechanisms responsible, gene targeting was used to study long-distance gene regulation. Remarkably, these targeted cell lines lost the translocated chromosome (t(11;14)). In these MCL and MM cells, the nonrearranged cyclin D1 (CCND1) locus reverts from CpG hypomethylated to hypermethylated. Reintroduction of the translocated chromosome induced a loss of methylation at the unrearranged CCND1 locus, providing evidence of a transallelic regulatory effect. In these cell lines and primary MCL patient samples, the CCND1 loci are packaged in chromatin-containing CCCTC binding factor (CTCF) and nucleophosmin (NPM) at the nucleolus. We show that CTCF and NPM are bound at the IgH 3′ regulatory elements only in the t(11;14) MCL cell lines. Furthermore, NPM short hairpin RNA produces a specific growth arrest in these cells. Our data demonstrate transvection in human cancer and suggest a functional role for CTCF and NPM.
登录
查看更多内容
DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
影响因子：
20.3
作者：
Fu, K;Weisenburger, DD;Chan, WC
通讯作者：
Chan, WC
影响因子：
5.3
作者：
Garrett, FE;Emelyanov, AV;Birshtein, BK
通讯作者：
Birshtein, BK
影响因子：
4.8
作者：
Hatada, I;Nabetani, A;Mukai, T
通讯作者：
Mukai, T
影响因子：
5.3
作者：
Boulon, S;Dantonel, JC;Philips, A
通讯作者：
Philips, A