Antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga Bryothamnion triquetrum.
Antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga Bryothamnion triquetrum.
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红色藻类Bryothamnion Triquetrum的粗甲醇提取物的抗伤害感受和抗炎活性。
DOI：
10.3390/md10091977
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发表时间：
2012-09
期刊：
影响因子：
5.4
通讯作者：
Alexandre-Moreira MS
中科院分区：
文献类型：
--
作者：
Cavalcante-Silva LHA;Barbosa Brito da Matta C;De Araújo MV;Barbosa-Filho JM;Pereira de Lira D;De Oliveira Santos BV;De Miranda GEC;Alexandre-Moreira MS
海洋环境是生物活性天然产品的非凡储藏库，其中许多具有陆地天然产品所没有的化学和结构特征。在这方面，本研究的目的是调查可能的抗伤害和抗炎活性的粗甲醇提取物的红毛藓三角（BT-MeOH）在小鼠模型。在整个实验过程中使用了两种性别的瑞士小鼠组（25-30 g）。通过以下试验评价BT-MeOH的潜在抗伤害性：乙酸诱导的扭体、热板试验和谷氨酸和福尔马林诱导的伤害性感受。采用酵母多糖A诱导的腹膜炎试验研究BT-MeOH的抗炎活性。使用100 mg/kg（p.o.）BT-MeOH，33.3 mg/kg（p.o.）安乃近35.7 mg/kg（p.o.）吲哚美辛和5.7 mg/kg（s.c.）吗啡在伤害性/炎症刺激前40分钟给予提取物和所有标准药物。在醋酸扭体实验中，BT-MeOH和安乃近分别抑制55.9%（22.2 ± 2.0次扭体; p < 0.01）和80.9%（9.6 ± 2.1次扭体; p < 0.01）的伤害性反应。在热板试验中，BT-MeOH在评价的时间内未增加动物的潜伏期。此外，BT-MeOH抑制谷氨酸诱导的伤害性感受50.1%。虽然BT-MeOH不抑制福尔马林诱导的伤害感受中的神经发生期，但炎症期被抑制53.1%（66.8 ± 14.2 s; p < 0.01）。消炎痛对炎症期的抑制率为60.2%（56.8 ± 8.7s; p < 0.01）。在酵母多糖诱导的腹膜炎试验中，BT-MeOH抑制55.6%（6.6 ± 0.2 × 106白细胞/mL; p < 0.01）的白细胞迁移，而吲哚美辛抑制78.1%（3.2 ± 0.1 × 106白细胞/mL; p < 0.01）。基于本研究中获得的结果，我们得出结论，BT-MeOH具有外周镇痛和抗炎活性。然而，需要进行更多的研究来证实这些特性。
The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities of a crude methanolic extract of the red alga Bryothamnion triquetrum (BT-MeOH) in murine models. Groups of Swiss mice of both sexes (25–30 g) were used throughout the experiments. The potential antinociceptive of BT-MeOH was evaluated by means of the following tests: acetic acid-induced writhing, hot-plate test and glutamate- and formalin-induced nociception. The anti-inflammatory activity of BT-MeOH was investigated using the zymosan A-induced peritonitis test. The tests were conducted using 100 mg/kg (p.o.) BT-MeOH, 33.3 mg/kg (p.o.) dipyrone, 35.7 mg/kg (p.o.) indomethacin and 5.7 mg/kg (s.c.) morphine. The extract and all standard drugs were administered 40 min before the nociceptive/inflammatory stimulus. In the acetic acid-induced writhing test, BT-MeOH and dipyrone inhibited the nociceptive response by 55.9% (22.2 ± 2.0 writhings; p < 0.01) and 80.9% (9.6 ± 2.1 writhings; p < 0.01). In the hot-plate test, BT-MeOH did not increase the latency time of the animals in the time evaluated. In addition, BT-MeOH inhibited glutamate-induced nociception by 50.1%. While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01). Indomethacin inhibited the inflammatory phase by 60.2% (56.8 ± 8.7 s; p < 0.01). In the zymosan-induced peritonitis test, BT-MeOH inhibited 55.6% (6.6 ± 0.2 × 106 leukocytes/mL; p < 0.01) of leukocyte migration, while indomethacin inhibited 78.1% (3.2 ± 0.1 × 106 leukocytes/mL; p < 0.01). Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities. However, more studies need to be conducted to confirm these properties.
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影响因子：
5.6
作者：
Bezerra-Santos, Claudio R.;Vieira-de-Abreu, Adriana;Bozza, Patricia T.
通讯作者：
Bozza, Patricia T.
DOI：
10.1590/s0102-695x2011005000079
发表时间：
2011-04-01
期刊：
Revista Brasileira de Farmacognosia
影响因子：
--
作者：
Paula, Joel Campos De;Vallim, Magui A.;Teixeira, Valéria Laneuville
通讯作者：
Teixeira, Valéria Laneuville
影响因子：
3.5
作者：
Chatter, R.;Cenac, N.;Vergnolle, N.
通讯作者：
Vergnolle, N.
影响因子：
7.3
作者：
COLLIER, HOJ;DINNEEN, LC;SCHNEIDER, C
通讯作者：
SCHNEIDER, C
影响因子：
5.1
作者：
de Lima, Flavia Oliveira;Nonato, Fabiana Regina;Villarreal, Cristiane Flora
通讯作者：
Villarreal, Cristiane Flora