A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand.
A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand.
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DOI：
10.1371/journal.pntd.0000056
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发表时间：
2007-10-31
影响因子：
3.8
通讯作者：
Peacock SJ
中科院分区：
文献类型：
--
作者：
Thaipadungpanit J;Wuthiekanun V;Chierakul W;Smythe LD;Petkanchanapong W;Limpaiboon R;Apiwatanaporn A;Slack AT;Suputtamongkol Y;White NJ;Feil EJ;Day NP;Peacock SJ
1999年至2003年间，泰国东北部持续爆发钩端螺旋体病，但爆发原因不明。 2000 年至 2005 年间进行了一项前瞻性研究，旨在识别泰国东北部乌隆他尼医院的钩端螺旋体病患者，并从血液中分离出致病微生物。开发了多位点序列分型方案来对这些致病性钩端螺旋体进行基因分型。对同期从泰国其他省份的人类病例以及 2004 年在东北部捕获的啮齿动物中分离出的钩端螺旋体进行了额外的分型。将序列类型 (ST) 与从参考收集中提取的钩端螺旋体进行了比较。在乌隆他尼患者的 101 例分离株中鉴定出 12 种 ST。其中一种 (ST34) 占分离株的 77 个 (76%)。 ST34是问号钩端螺旋体，秋季血清型。 2000/2001 年，乌隆他尼的人类钩端螺旋体分离株中有 86% 对应于 ST34，但随着疫情的减弱，这一数字到 2005 年下降到 56% (p = 0.01)。 ST34 代表来自泰国其他省份的人类分离株的 17/24 (71%) 和啮齿动物分离株的 7/8 (88%)。相比之下，在 76 个参考菌株中发现了 59 个 ST，表明种群遗传结构更加多样化； ST34 在此集合中未被识别。针对问号钩端螺旋体的 MLST 方案的制定表明，问号钩端螺旋体的单一生态成功致病性克隆在啮齿动物群体中占主导地位，并且与泰国人类钩端螺旋体病的持续爆发有关。 1999年至2003年间，泰国东北部持续爆发人类钩端螺旋体病，但爆发原因不明。钩端螺旋体病是一种由称为钩端螺旋体的细菌引起的潜在严重感染。感染通常发生在环境暴露于受感染动物尿液中排出的致病性钩端螺旋体之后。本研究的目的是在泰国爆发期间从患有钩端螺旋体病的人类身上获得细菌分离物，以进行基因分型，并将其与维持宿主动物联系起来。为了实现这一目标，我们针对人类疾病的主要原因问号钩菌开发了细菌分型方案（多位点序列分型，MLST）。这种方法比现有的打字方案具有优势，因为生成的数据适合详细的进化分析，并且可以通过互联网轻松进行比较。我们的结果证明了问询钩端螺旋体 Autumnalis 的显性克隆的出现；这是爆发期间人类疾病的主要原因，并在被定义为袋狸的维持宿主中发现。
A sustained outbreak of leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown. A prospective study was conducted between 2000 and 2005 to identify patients with leptospirosis presenting to Udon Thani Hospital in northeast Thailand, and to isolate the causative organisms from blood. A multilocus sequence typing scheme was developed to genotype these pathogenic Leptospira. Additional typing was performed for Leptospira isolated from human cases in other Thai provinces over the same period, and from rodents captured in the northeast during 2004. Sequence types (STs) were compared with those of Leptospira drawn from a reference collection. Twelve STs were identified among 101 isolates from patients in Udon Thani. One of these (ST34) accounted for 77 (76%) of isolates. ST34 was Leptospira interrogans, serovar Autumnalis. 86% of human Leptospira isolates from Udon Thani corresponded to ST34 in 2000/2001, but this figure fell to 56% by 2005 as the outbreak waned (p = 0.01). ST34 represented 17/24 (71%) of human isolates from other Thai provinces, and 7/8 (88%) rodent isolates. By contrast, 59 STs were found among 76 reference strains, indicating a much more diverse population genetic structure; ST34 was not identified in this collection. Development of an MLST scheme for Leptospira interrogans revealed that a single ecologically successful pathogenic clone of L. interrogans predominated in the rodent population, and was associated with a sustained outbreak of human leptospirosis in Thailand. A sustained outbreak of human leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown. Leptospirosis is a potentially serious infection cause by bacteria known as Leptospira; infection usually occurs following environmental exposure to pathogenic Leptospira shed in the urine of an infected animal. The purpose of this study was to obtain bacterial isolates from humans with leptospirosis around the time of the Thai outbreak for genotyping, and to relate these to the maintenance host animal. To achieve this, a bacterial typing scheme (multilocus sequence typing, MLST) was developed for L. interrogans, the major cause of human disease. This approach has the advantage over existing typing schemes in that the data generated are amenable to detailed evolutionary analysis, and are readily comparable via the internet. Our results demonstrated the emergence of a dominant clone of L. interrogans serovar Autumnalis; this was the major cause of human disease during the outbreak, and was found in a maintenance host which was defined as the bandicoot rat.
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影响因子：
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