Are the meiofaunal polychaetes Hesionides arenaria and Stygocapitella subterranea true cosmopolitan species? — results of RAPD‐PCR investigations
Are the meiofaunal polychaetes Hesionides arenaria and Stygocapitella subterranea true cosmopolitan species? — results of RAPD‐PCR investigations
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小型多毛类Hesionides arenararia 和Stygocapitella subterranea 是真正的世界性物种吗——RAPD-PCR 调查结果
DOI：
10.1046/j.1463-6409.2000.00026.x
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发表时间：
2000
影响因子：
2.5
通讯作者：
W. Westheide
中科院分区：
文献类型：
--
作者：
H. Schmidt;W. Westheide
根据世界各地不同地点的记录，沙滩的间隙小型底栖动物包括许多被视为世界性动物的物种。关于这种分布的原因存在着长期的争论，但对于大多数这些物种来说，它们实际上是真正的物种还是“仅仅”是神秘物种的复合体仍然存在疑问。在这里，遗传分化研究内和两个物种之间被认为是典型的世界性，Hesionides arenaria arenaria（Hesionidae）和Stygocapitella subdicherea（Parergodrilidae），通过RAPD-PCR分析标本来自两大洲的不同海洋区域。对于鼠伤寒沙门氏用14条引物对北海沿岸（Sylt）、美国大西洋沿岸（马萨诸塞州）和太平洋沿岸（华盛顿）的3个地点的subducea进行了DNA扩增，共发现335个不同的DNA片段，其中20个为欧洲动物的诊断片段，17个为美国东海岸的动物的诊断片段，14个为美国西海岸的动物的诊断片段。五个聚类程序，所有这些都显着区分三个种群作为单独的遗传分支，建议每个物种的地位。而H. a. arenaria从8个欧洲网站之间的斯卡格拉克和地中海东部（包括加那利群岛），和北美太平洋沿岸，其中468个不同的DNA片段扩增12个引物，不形成单独的遗传分支。因为没有一个群体能够证明哪怕一个诊断特征。因此，H. a.已在DNA水平上得到证实。
The interstitial meiofauna of sand beaches includes many species that are regarded as cosmopolites, on the basis of records from various sites throughout the world. There is a long‐standing debate about the causes of such a distribution, but for most of these species it is still questionable whether they are in fact true species or ‘merely’ complexes of cryptic species. Here genetic differentiation is examined within and between two species considered typically cosmopolitan, Hesionides arenaria arenaria (Hesionidae) and Stygocapitella subterranea (Parergodrilidae), by RAPD‐PCR analyses of specimens from diverse marine regions on two continents. For S. subterranea at three sites, on the North Sea coast (Sylt) and the US Atlantic (Massachusetts) and Pacific (Washington) coasts, with 14 primers 335 different DNA fragments were found: 20 diagnostic ones for the European animals, 17 for the animals from the American East Coast and 14 for those from the American West Coast. Five cluster procedures were used, all of which significantly distinguished the three populations as separate genetic clades; it is recommended that each be given species status. In contrast, the individual specimens of H. a. arenaria from eight European sites, between Skagerrak and the eastern Mediterranean (including the Canary Islands), and the North American Pacific coast, for which 468 different DNA fragments were amplified with 12 primers, do not form separate genetic clades. For no single population was it possible to demonstrate even one diagnostic character. Therefore the cosmopolitan nature of H. a. arenaria has been confirmed at the DNA level.
蚊子种类和种群（双翅目：蚊科）的随机扩增多态性 DNA：技术、统计分析和应用。
影响因子：
2.1
作者：
Kambhampati,S;Black4th,WC;Rai,KS
通讯作者：
Rai,KS