Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample.
Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample.
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DOI：
10.1007/s13361-018-1933-y
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发表时间：
2018-06
影响因子：
3.2
通讯作者：
Novotny MV
中科院分区：
文献类型：
--
作者：
Benktander JD;Gizaw ST;Gaunitz S;Novotny MV
糖缀合物直接或间接参与许多生物过程。由于其复杂的结构，聚糖的结构解析及其在生物系统中的作用的探索一直具有挑战性。通过从糖蛋白或糖脂混合物释放产生的聚糖池通常非常复杂。为了程序的简单性，许多聚糖谱研究选择集中于单一类别的糖缀合物。在本文中，我们证明了从同一样品中分离的鞘糖脂、N-聚糖和O-聚糖是可行的。小体积的人血清和腹水以及小的小鼠脑组织样品足以对来自所有三类糖缀合物的聚糖进行连续分析，甚至通过MALDI-MS和LC-ESI-MS对一些混合物组分进行阳性鉴定。结果表明，从相当于500 μg蛋白质起始材料或可能更少的蛋白质起始材料可以获得全面的聚糖谱。加速未来的糖组学综合研究，特别是对珍贵的临床样本。
Glycoconjugates are directly or indirectly involved in many biological processes. Due to their complex structures, the structural elucidation of glycans and the exploration of their role in biological systems have been challenging. Glycan pools generated through release from glycoprotein, or glycolipid mixtures can often be very complex. For the sake of procedural simplicity, many glycan profiling studies choose to concentrate on a single class of glycoconjugates. In this paper, we demonstrate it feasible to cover glycosphingolipids, N-glycans and O-glycans isolated from the same sample. Small volumes of human blood serum and ascites fluid as well as small mouse brain tissue samples are sufficient to profile sequentially glycans from all three classes of glycoconjugates and even positively identify some mixture components through MALDI-MS and LC-ESI-MS. The results show that comprehensive glycan profiles can be obtained from the equivalent of 500 μg protein starting material or possibly less. These methodological improvements can help accelerating future glycomic comprehensive studies, especially for precious clinical samples.
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影响因子：
7.4
作者：
Gaunitz S;Nagy G;Pohl NL;Novotny MV
通讯作者：
Novotny MV
影响因子：
7.4
作者：
Alley, William R., Jr.;Madera, Milan;Mechref, Yehia;Novotny, Milos V.
通讯作者：
Novotny, Milos V.
影响因子：
7.4
作者：
Goetz, John A.;Novotny, Milos V.;Mechref, Yehia
通讯作者：
Mechref, Yehia
DOI：
10.1016/j.jchromb.2013.11.025
发表时间：
2014-02-01
影响因子：
3
作者：
Garcia, Aldo D.;Chavez, Jorge L.;Mechref, Yehia
通讯作者：
Mechref, Yehia
DOI：
10.1074/mcp.m111.011601
发表时间：
2012-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Balog CI;Stavenhagen K;Fung WL;Koeleman CA;McDonnell LA;Verhoeven A;Mesker WE;Tollenaar RA;Deelder AM;Wuhrer M
通讯作者：
Wuhrer M