Purification and Cloning of an Apoptosis-Inducing Protein Derived from Fish Infected with Anisakis simplex, a Causative Nematode of Human Anisakiasis1
Purification and Cloning of an Apoptosis-Inducing Protein Derived from Fish Infected with Anisakis simplex, a Causative Nematode of Human Anisakiasis1
复制标题
从感染单纯异尖线虫（人类异尖线虫病的一种线虫）的鱼中提取的凋亡诱导蛋白的纯化和克隆1
作者：
Sang;A. Mai;M. Iwamoto;N. Arizono;D. Fujimoto;K. Sakamaki;S. Yonehara
在研究海产品对细胞生长的影响时，我们发现海鱼内脏提取物对多种哺乳动物肿瘤细胞具有强大的诱导凋亡作用，并呈剂量依赖关系。这种活性显著地依赖于感染单纯异尖线虫幼虫的鲱鱼。在纯化了具有诱导凋亡活性的蛋白后，我们克隆了相应的基因，发现它是一个黄素蛋白。该蛋白被称为凋亡诱导蛋白(AIP)，还具有内质网滞留信号(C-末端KDEL序列)和产生过氧化氢的活性，表明我们分离到了一个新的具有诱导细胞凋亡活性的网织蛋白。AIP只有在感染幼虫线虫后才能在鱼体内诱导，并定位于幼虫周围形成的囊膜上，以防止它们迁移到宿主组织。我们的结果表明，AIP可能具有阻止线虫感染的功能。
While investigating the effect of marine products on cell growth, we found that visceral extracts of Chub mackerel, an ocean fish, had a powerful and dose-dependent apoptosis-inducing effect on a variety of mammalian tumor cells. This activity was strikingly dependent on infection of the C. mackerel with the larval nematode, Anisakis simplex. After purification of the protein responsible for the apoptosis-inducing activity, we cloned the corresponding gene and found it to be a flavoprotein. This protein, termed apoptosis-inducing protein (AIP), was also found to possess an endoplasmic reticulum retention signal (C-terminal KDEL sequence) and H2O2-producing activity, indicating that we had isolated a novel reticuloplasimin with potent apoptosis-inducing activity. AIP was induced in fish only after infection with larval nematode and was localized to capsules that formed around larvae to prevent their migration to host tissues. Our results suggest that AIP may function to impede nematode infection.
DOI：
10.1073/pnas.88.13.5513
发表时间：
1991-07-01
影响因子：
11.1
作者：
URBAN, JF;KATONA, IM;FINKELMAN, FD
通讯作者：
FINKELMAN, FD
蛇毒L-氨基酸氧化酶的一级结构与小鼠B细胞白细胞介素4诱导的Fig1蛋白具有高度同源性。
影响因子：
3.1
作者：
Raibekas,AA;Massey,V
通讯作者：
Massey,V