Characterization of Epstein–Barr virus (EBV)‐infected natural killer (NK) cell proliferation in patients with severe mosquito allergy; establishment of an IL‐2‐dependent NK‐like cell line
Characterization of Epstein–Barr virus (EBV)‐infected natural killer (NK) cell proliferation in patients with severe mosquito allergy; establishment of an IL‐2‐dependent NK‐like cell line
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严重蚊子过敏患者中 Epstein-Barr 病毒 (EBV) 感染的自然杀伤 (NK) 细胞增殖的特征；IL-2 依赖性 NK 样细胞系的建立；
DOI：
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发表时间：
1999
影响因子：
4.6
通讯作者：
Hiroshi Matsuoka
中科院分区：
文献类型：
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作者：
Ikuya Tsuge;T. Morishima;M. Morita;Hiroshi Kimura;Kiyotaka Kuzushima;Hiroshi Matsuoka
蚊虫叮咬严重超敏反应（HMB）与EBV感染NK细胞克隆扩增之间关系的临床证据已经积累。为了阐明EBV诱导NK细胞增殖的机制及其与HMB患者白血病或淋巴瘤高发的关系，我们对3例HMB患者的NK细胞进行了克隆扩增，并成功建立了EBV感染的NK样细胞系KAI3。免疫印迹和逆转录聚合酶链反应（RT - PCR）分析显示，KAI3细胞和受感染的NK细胞表现出EBV潜伏感染II型，其中EBV基因表达仅限于EBNA 1和LMP1。KAI3通过IL - 2培养建立，我们检测了患者外周血NK细胞对IL - 2的反应性。结果表明，IL - 2诱导的IL - 2Rα在NK细胞中的表达明显增强。这种特性可能有助于感染NK细胞的持续扩增。然而，无论是抗Fas抗体还是NK敏感的K562细胞，患者的KAI3细胞和NK细胞都不能免受凋亡的保护。保留对凋亡的敏感性可能解释了患者外周血中相对调节的NK细胞数量。据我们所知，KAI3是第一个报道的从白血病或淋巴瘤发病前严重慢性活动性EBV感染（SCAEBV）患者中建立的NK样细胞系。KAI3细胞将有助于研究EBV在NK细胞环境中的持续性及其与HMB患者白血病或淋巴瘤高发的关系。
The clinical evidence of a relationship between severe hypersensitivity to mosquito bite (HMB) and clonal expansion of EBV‐infected NK cells has been accumulated. In order to clarify the mechanism of EBV‐induced NK cell proliferation and its relationship with high incidence of leukaemias or lymphomas in HMB patients, we studied clonally expanded NK cells from three HMB patients and succeeded in establishing an EBV‐infected NK‐like cell line designated KAI3. Immunoblotting and reverse transcriptase‐polymerase chain reaction (RT‐PCR) analyses revealed that KAI3 cells as well as infected NK cells exhibited an EBV latent infection type II, where EBV gene expression was limited to EBNA 1 and LMP1. As KAI3 was established by culture with IL‐2, IL‐2 responsiveness of peripheral blood NK cells from patients was examined. The results represented markedly augmented IL‐2‐induced IL‐2Rα expression in NK cells. This characteristic property may contribute to the persistent expansion of infected NK cells. However, KAI3 cells as well as the NK cells from patients were not protected from apoptosis induced by either an anti‐Fas antibody or NK‐sensitive K562 cells. Preserved sensitivity to apoptosis might explain the relatively regulated NK cell numbers in the peripheral blood of the patients. To our knowledge, KAI3 is the first reported NK‐like cell line established from patients of severe chronic active EBV infection (SCAEBV) before the onset of leukaemias or lymphomas. KAI3 cells will contribute to the study of EBV persistency in the NK cell environment and its relationship with high incidence of leukaemias or lymphomas in HMB patients.
病毒诱导的淋巴细胞增殖的控制：EB病毒诱导的淋巴细胞增殖和宿主免疫。
DOI：
10.1016/s1357-4310(96)10054-x
发表时间：
1997
期刊：
Molecular medicine today.
影响因子：
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作者：
Rooney,CM;Smith,CA;Heslop,HE
通讯作者：
Heslop,HE