Alteration in information flow through a pair of feeding command neurons underlies a form of Pavlovian conditioning in the Drosophila brain.
Alteration in information flow through a pair of feeding command neurons underlies a form of Pavlovian conditioning in the Drosophila brain.
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DOI：
10.1016/j.cub.2021.07.021
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发表时间：
2021-09-27
期刊：
影响因子：
9.2
通讯作者：
Yoshihara, Motojiro
中科院分区：
文献类型：
--
作者：
Sakurai, Akira;Littleton, J. Troy;Kojima, Hiroaki;Yoshihara, Motojiro
巴甫洛夫条件反射是一种广泛使用的学习范式，其中定义的刺激相关联，以诱导行为转换。为了确定单个神经元的活动变化与行为转换之间的因果关系，我们利用了一个将细胞功能与行为联系起来的“命令神经元”。为了研究巴甫洛夫条件反射的细胞和分子基础，我们以前确定了一对喂养命令神经元称为“喂养神经元”在成年果蝇大脑中使用遗传筛选和opto/thermogenetic技术。摄食神经元被甜味信号（如蔗糖）激活，并诱导完整的摄食行为，如长鼻伸展和食物泵送。切除或失活这对摄食神经元可以消除摄食行为，这表明这对神经元对自然摄食行为是不可或缺的。在这里，我们描述了一种新的条件协议相关联的信号介导杆从腿（条件刺激; CS）蔗糖刺激（无条件刺激; US）诱导的摄食行为。摄食神经元的钙成像表明，它在条件反射过程中获得对CS的反应性，条件反射过程中摄食神经元的失活抑制可塑性。这些结果表明，条件反射改变了从CS流入摄食回路的信号，摄食神经元作为Hebbian可塑性的关键整合枢纽发挥作用。
Pavlovian conditioning is a broadly used learning paradigm where defined stimuli are associated to induce behavioral switching. To define a causal relationship between activity change in a single neuron and behavioral switching, we took advantage of a “command neuron” that connects cellular function to behavior. To examine the cellular and molecular basis of Pavlovian conditioning, we previously identified a pair of feeding command neurons termed “Feeding neurons” in the adult Drosophila brain using genetic screening and opto/thermogenetic techniques. The Feeding neuron is activated by sweet signals like sucrose and induces the full complement of feeding behaviors such as proboscis extension and food pumping. Ablation or inactivation of the pair of Feeding neurons abolishes feeding behavior, suggesting this single pair of neurons is indispensable for natural feeding behaviors. Here we describe a novel conditioning protocol to associate a signal mediating rod removal from legs (conditioned stimulus; CS) to feeding behavior induced by sucrose stimulation (unconditioned stimulus; US). Calcium imaging of the Feeding neuron demonstrated it acquires responsiveness to CS during conditioning, with inactivation of the Feeding neuron during conditioning suppressing plasticity. These results suggest conditioning alters signals flowing from the CS into the feeding circuit, with the Feeding neuron functioning as a key integrative hub for Hebbian plasticity.
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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