Early effects of parathyroid hormone on membrane potential of rat osteoblasts in culture: Role of cAMP and Ca2+
Early effects of parathyroid hormone on membrane potential of rat osteoblasts in culture: Role of cAMP and Ca2+
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甲状旁腺激素对培养的大鼠成骨细胞膜电位的早期影响：cAMP 和 Ca2+ 的作用
DOI：
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发表时间：
1988
影响因子：
6.2
通讯作者：
S. Balsan
中科院分区：
文献类型：
--
作者：
Jeanine Fritsch;A. Edelman;S. Balsan
应用微电极技术研究了cAMP和Ca ~（2+）离子参与甲状旁腺激素（bPTH）（1-34）对原代培养大鼠成骨细胞膜电位的影响。甲状旁腺激素（10−7 M）使细胞膜去极化25.0 ± 6.1 mV（平均值±标准差，SD; n = 17）。用钙通道阻断剂钴阻断钙内流显示激素效应的两个阶段：快速和轻微的膜超极化，然后持续去极化。此外，钴显著（p < 0.01）降低PTH去极化作用的幅度。向灌注液中加入二丁酰-cAMP（10−3 M）也导致了双相效应。在较低浓度（10−4 M）下，二丁酰cAMP仅产生膜超极化，表明相反膜电位变化的cAMP剂量依赖性。毛喉素（10−5 M）和磷酸二酯酶抑制剂异丁基甲基黄嘌呤（IBMX）（10−4 M）模拟PTH的去极化作用。低浓度（5 × 10−6 M）的IBMX可增强PTH的去极化作用。使用Ca 2+离子载体A23187和细胞内注射CaCl 2或三磷酸肌醇增加[Ca 2 +]i降低了PTH去极化作用，而细胞内注射EGTA增强了这种作用。这些结果表明，甲状旁腺素引起大鼠成骨细胞膜电位的双相变化，这似乎是介导的cAMP的增加和细胞内钙的调制。
Microelectrodes were used to investigate the possible involvement of cAMP and Ca2+ ions in the parathyroid hormone's, bPTH(1–34), effect on the membrane potential of rat osteoblasts in primary culture. Parathyroid hormone (10−7 M) depolarized cell membrane by 25.0 ± 6.1 mV (mean ± standard deviation, SD; n = 17). Blocking Ca2+ influx with the Ca channel blocker cobalt revealed two phases in the hormone effect: a rapid and slight membrane hyperpolarization followed by sustained depolarization. In addition, cobalt significantly (p < 0.01) decreased the magnitude of the PTH depolarizing action. The addition of dibutyryl‐cAMP (10−3 M) to the perfusion solution also resulted in a biphasic effect. At a lower concentration (10−4 M), dibutyryl‐cAMP produced only membrane hyperpolarization, suggesting a cAMP dose dependence of the opposite membrane potential changes. Forskolin (10−5 M) and the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) (10−4 M) mimicked the depolarizing effect of PTH. IBMX at a low concentration (5 × 10−6 M) potentiated the depolarizing effect of PTH. Increases in [Ca2+]i using Ca2+ ionophore A23187 and intracellular injection of CaCl2 or inositol trisphosphate decreased the PTH depolarizing action, whereas intracellular injection of EGTA enhanced this effect. These results indicate that PTH evokes a biphasic change in rat osteoblast membrane potential that seems to be mediated by an increase in cAMP and modulated by intracellular calcium.
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DOI：
10.1152/ajprenal.1986.251.2.f188
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Hruska,KA;Goligorsky,M;Scoble,J;Tsutsumi,M;Westbrook,S;Moskowitz,D
通讯作者：
Moskowitz,D
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DOI：
10.1172/jci112788
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Hruska,KA;Moskowitz,D;Esbrit,P;Civitelli,R;Westbrook,S;Huskey,M
通讯作者：
Huskey,M
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DOI：
10.1152/ajpendo.1987.253.1.e45
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Reid,IR;Civitelli,R;Halstead,LR;Avioli,LV;Hruska,KA
通讯作者：
Hruska,KA
犬肾 PTH 受体环化酶系统的调节：钙、镁和鸟嘌呤核苷酸的影响。
DOI：
10.1152/ajprenal.1981.241.4.f364
发表时间：
1981
期刊：
The American journal of physiology
影响因子：
--
作者：
Bellorin-Font,E;Martin,KJ
通讯作者：
Martin,KJ