Alteration in expression of smooth muscle alpha-actin associated with transformation of rat 3Y1 cells.
Alteration in expression of smooth muscle alpha-actin associated with transformation of rat 3Y1 cells.
复制标题
与大鼠 3Y1 细胞转化相关的平滑肌 α-肌动蛋白表达的变化。
作者：
M. Okamoto;S. Taniguchi;H. Sadano;T. Kawano;G. Kimura;G. Gabbiani;T. Baba
Expression of actin was examined in a cultured rat embryonic cell line 3Y1 and transformed cell lines that originated from 3Y1. An alpha-actin in addition to cytoplasmic beta- and gamma-actins was detected in 3Y1 by two-dimensional gel electrophoresis. This alpha-actin was hardly detected at all in the transformants induced by Rous sarcoma virus, v-H-ras oncogene or adenovirus type 12, while the alpha-actin was retained in the transformed cell lines induced by N-methyl-N'-nitro-N-nitrosoguanidine or in SV40, which are cell lines of relatively low malignancy. Western and Northern blot analyses established that this alpha-actin was a smooth muscle alpha-isoform. An immunofluorescence study revealed that smooth muscle alpha-actin in 3Y1 cells is present in stress fibers. Thus, smooth muscle alpha-actin is also a component of actin stress fibers, as beta- and gamma-actins are in 3Y1 cells. An alteration in the expression of this actin isoform may be related to phenotypical changes accompanying transformation.
从正常和转化的大鼠培养细胞中分离的微丝中原肌球蛋白的差异表达形式。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Matsumura,F;Lin,JJ;Yamashiro-Matsumura,S;Thomas,GP;Topp,WC
通讯作者：
Topp,WC
DOI：
10.1073/pnas.81.23.7476
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ELDER, PK;SCHMIDT, LJ;GETZ, MJ
通讯作者：
GETZ, MJ
影响因子：
11.4
作者：
LAU, LF;NATHANS, D
通讯作者：
NATHANS, D