Divergent Effects of Activated Neutrophils on Inflammation, Kupffer Cell/Splenocyte Activation, and Lung Injury Following Blunt Chest Trauma
Divergent Effects of Activated Neutrophils on Inflammation, Kupffer Cell/Splenocyte Activation, and Lung Injury Following Blunt Chest Trauma
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活化的中性粒细胞对胸部钝挫伤后炎症、库普弗细胞/脾细胞活化和肺损伤的不同影响
DOI：
10.1097/shk.0b013e31823f1776
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发表时间：
2012
期刊：
影响因子：
3.1
通讯作者：
Knöferl MW
中科院分区：
文献类型：
--
作者：
Perl M;Kieninger M;Huber-Lang M;Gross HJ;Bachem MG;Braumüller S;Gebhard F;Ayala A;Knöferl MW
多形核粒细胞（PMNs）被认为在急性肺损伤（ALI）的发病机制中起着主要的有害作用。然而，有证据表明，pmn也可能对某些类型的ALI有益。在这方面，我们研究了活化中性粒细胞在肺挫伤诱发ALI的病理生理中的作用。我们采用雄性C3H/HeN小鼠钝性胸外伤伴pmn耗竭模型。动物在创伤前48 h静脉注射25 μg/g体重的pmn消耗抗体Gr-1。创伤后，支气管肺泡灌洗（BAL）和肺组织白细胞介素6 （IL-6）在pmn缺失和对照动物中同样升高，而BAL和肺中的巨噬细胞炎症蛋白2和单核细胞趋化蛋白1，BAL中的IL-10和肺角化细胞趋化剂（KC）在pmn缺失的情况下甚至进一步升高。血浆IL-6和KC也在损伤后升高，在没有PMNs的情况下甚至进一步升高。胸外伤诱导离体KU中IL-6、肿瘤坏死因子α、巨噬细胞炎症蛋白2、单核细胞趋化蛋白1和IL-10的释放增强，在没有PMNs的情况下，IL-6释放减弱。在PMNs存在的情况下，与3 h时间点相比，BAL蛋白在30 h时进一步升高，而在没有PMNs存在的情况下则没有这种情况。综上所述，在对肺创伤的反应中，活化的中性粒细胞控制炎症，包括远端免疫细胞释放介质，但同时介导肺组织损伤。因此，考虑到潜在的炎症不良反应，调节中性粒细胞的激活或运输可能是胸外伤性肺损伤的合理治疗方法。
Polymorphonuclear granulocytes (PMNs) have been attributed a primarily deleterious role in the pathogenesis of acute lung injury (ALI). However, evidence exists that PMNs might also act beneficially in certain types of ALI. In this regard, we investigated the role of activated neutrophils in the pathophysiology of lung contusion–induced ALI. We used the model of blunt chest trauma accompanied by PMN-depletion in male C3H/HeN mice. Animals received 25 μg/g body weight PMN-depleting antibody Gr-1 intravenously 48 h before trauma. Bronchoalveolar lavage (BAL) and lung tissue interleukin 6 (IL-6) were similarly elevated in PMN-depleted and control animals after trauma, whereas macrophage inflammatory protein 2 and monocyte chemoattractant protein 1 in BAL and lungs, IL-10 in BAL, and lung keratinocyte chemoattractant (KC) were even further increased in the absence of PMNs. Plasma IL-6 and KC were also increased in response to the insult and even further in the absence of PMNs. Chest trauma induced an enhanced release of IL-6, tumor necrosis factor α, macrophage inflammatory protein 2, monocyte chemoattractant protein 1, and IL-10 from isolated KU, which was blunted in the absence of PMNs. In the presence of PMNs, BAL protein was further increased at 30 h when compared with the 3-h time point, which was not the case in the absence of PMNs. Taken together, in response to lung trauma, activated neutrophils control inflammation including mediator release from distant immune cells but simultaneously mediate pulmonary tissue damage. Thus, keeping in mind potential inflammatory adverse effects, modulation of neutrophil activation or trafficking might be a reasonable therapeutic approach in chest trauma–induced lung injury.
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肺部创伤。
DOI：
--
发表时间：
1997
期刊：
Chest Surgery Clinics of North America
影响因子：
--
作者：
A. Boyd;L. Glassman
通讯作者：
L. Glassman
影响因子：
15.9
作者：
Yanase, K;Smith, RM;Madaio, MP
通讯作者：
Madaio, MP
人类体外循环期间中性粒细胞粘附分子的差异模式。
影响因子：
37.8
作者：
M. Galiñanes;C. Watson;U. Trivedi;D. Chambers;C. Young;G. Venn
通讯作者：
G. Venn
影响因子：
8.8
作者：
Perl, M;Gebhard, F;Knöferl, MW
通讯作者：
Knöferl, MW
影响因子：
3.1
作者：
Knöferl, MW;Liener, UC;Gebhard, F
通讯作者：
Gebhard, F