The DNA damage response: the omics era and its impact.
The DNA damage response: the omics era and its impact.
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DOI：
10.1016/j.dnarep.2014.03.008
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发表时间：
2014-07
期刊：
影响因子：
3.8
通讯作者：
Pothof, Joris
中科院分区：
文献类型：
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作者：
Derks, Kasper W. J.;Hoeijmakers, Jan H. J.;Pothof, Joris
监测基因组、转录组和蛋白质组与遗传毒性应激相关的高密度技术的出现极大地增强了我们对DNA损伤反应中的全球反应和动态的了解，包括它与癌症和衰老的关系。此外，“组学”技术在细胞对DNA损伤的反应中发现了许多新的因素，它们的翻译后修饰、途径和全球反应。基于组学，目前估计有数以千计的基因(产物)S参与DNA损伤反应，识别在最宝贵的细胞分子DNA损伤后决定细胞命运的复杂网络。下一代测序技术和相关专门技术的发展可以以前所未有的单核苷酸分辨率定量监测RNA和DNA。在这篇综述中，我们将讨论组学技术，特别是下一代测序技术对我们理解DNA损伤反应的贡献，以及下一代测序的未来前景，它的单细胞应用和组学数据集集成在解开复杂的DNA损伤信号网络中的应用。
The emergence of high density technologies monitoring the genome, transcriptome and proteome in relation to genotoxic stress have tremendously enhanced our knowledge on global responses and dynamics in the DNA damage response, including its relation with cancer and aging. Moreover, ‘-omics’ technologies identified many novel factors, their post-translational modifications, pathways and global responses in the cellular response to DNA damage. Based on omics, it is currently estimated that thousands of gene(product)s participate in the DNA damage response, recognizing complex networks that determine cell fate after damage to the most precious cellular molecule, DNA. The development of next generation sequencing technology and associated specialized protocols can quantitatively monitor RNA and DNA at unprecedented single nucleotide resolution. In this review we will discuss the contribution of omics technologies and in particular next generation sequencing to our understanding of the DNA damage response and the future prospective of next generation sequencing, its single cell application and omics dataset integration in unraveling intricate DNA damage signaling networks.
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影响因子：
16
作者：
Beli, Petra;Lukashchuk, Natalia;Wagner, Sebastian A.;Weinert, Brian T.;Olsen, Jesper V.;Baskcomb, Linda;Mann, Matthias;Jackson, Stephen P.;Choudhary, Chunaram
通讯作者：
Choudhary, Chunaram
影响因子：
48
作者：
Crosetto, Nicola;Mitra, Abhishek;Silva, Maria Joao;Bienko, Magda;Dojer, Norbert;Wang, Qi;Karaca, Elif;Chiarle, Roberto;Skrzypczak, Magdalena;Ginalski, Krzysztof;Pasero, Philippe;Rowicka, Maga;Dikic, Ivan
通讯作者：
Dikic, Ivan
影响因子：
16
作者：
Guénolé A;Srivas R;Vreeken K;Wang ZZ;Wang S;Krogan NJ;Ideker T;van Attikum H
通讯作者：
van Attikum H
DOI：
10.3402/pba.v1i0.7219
发表时间：
2011-01-01
期刊：
PATHOBIOLOGY OF AGING AND AGE-RELATED DISEASES
影响因子：
--
作者：
Dolle, Martijn E. T.;Kuiper, Raoul V.;van Steeg, Harry
通讯作者：
van Steeg, Harry
影响因子：
64.8
作者：
通讯作者：
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