A novel NADP(+)-dependent dehydrogenase activity for 7alpha/beta- and 11beta-hydroxysteroids in human liver nuclei: A third 11beta-hydroxysteroid dehydrogenase.
A novel NADP(+)-dependent dehydrogenase activity for 7alpha/beta- and 11beta-hydroxysteroids in human liver nuclei: A third 11beta-hydroxysteroid dehydrogenase.
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人肝核中 7α/β- 和 11β-羟基类固醇的新型 NADP() 依赖性脱氢酶活性：第三种 11β-羟基类固醇脱氢酶。
DOI：
10.1016/j.abb.2009.04.010
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发表时间：
2009
影响因子：
3.9
通讯作者：
Prough,RA
中科院分区：
文献类型：
--
作者：
Robinzon,B;Prough,RA
Human tissue from uninvolved liver of cancer patients was fractionated using differential centrifugation and characterized for 11βHSD enzyme activity against corticosterone, dehydrocorticosterone, 7α- and 7β-hydroxy-dehydroepiandrosterone, and 7-oxo-dehydroepiandrosterone. An enzyme activity was observed in nuclear protein fractions that utilized either NADP+or NAD+, but not NADPH and NADH, as pyridine nucleotide cofactor with Kmvalues of 12±2 and 390±2μM, compared to the Kmfor microsomal 11βHSD1 of 43±8 and 264±24μM, respectively. The Kmfor corticosterone in the NADP+-dependent nuclear oxidation reaction was 102±16nM, compared to 4.3±0.8μM for 11βHSD1. The Kcatvalues for nuclear activity with NADP+was 1687nmol/min/mg/μmol, compared to 755nmol/min/mg/μmol for microsomal 11βHSD1 activity. Inhibitors of 11βHSD1 decreased both nuclear and microsomal enzyme activities, suggesting that the nuclear activity may be due to an enzyme similar to 11βHSD Type 1 and 2.
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作者：
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DOI：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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W. Song;W. Pierce;Y. Saeki;R. Redinger;R. Prough