Screen Key Genes Associated with Distraction-Induced Osteogenesis of Stem Cells Using Bioinformatics Methods.
Screen Key Genes Associated with Distraction-Induced Osteogenesis of Stem Cells Using Bioinformatics Methods.
复制标题
使用生物信息学方法，与分散注意力诱导的干细胞的成骨相关的屏幕键基因。
DOI：
10.3390/ijms22126505
复制
发表时间：
2021-06-17
影响因子：
5.6
通讯作者：
Song W
中科院分区：
文献类型：
--
作者：
Chen J;Hua J;Song W
背景资料：应用间充质干细胞（MSC），与牵引成骨（DO）过程，显示出增强的骨质量和更短的治疗时间。DO通过提供机械线索来指导MSC的分化。然而，潜在的关键基因和途径在很大程度上是未知的。本研究的目的是筛选和鉴定牵张诱导骨髓间充质干细胞成骨的hub基因及其可能的分子机制。材料和方法：数据集从ArrayExpress数据库下载。选取3个阴性对照样本和2个0.25 Hz 5%正弦牵张6 h的样本进行差异表达基因（DEG）筛选，并通过生物信息学方法进行分析。研究了基因本体论（GO）术语和京都基因和基因组百科全书（KEGG）途径富集。蛋白质-蛋白质相互作用（PPI）网络通过Cytoscape软件可视化。进行基因集富集分析（GSEA）以验证自定义的成骨基因集集合的富集并鉴定成骨枢纽基因。结果如下：通过Venn图确定了与牵张诱导的MSC成骨高度相关的三个枢纽基因（IL 6，MMP 2和EP 300）。这些枢纽基因可以提供一个新的理解，牵拉诱导的成骨分化的间充质干细胞，并作为潜在的基因靶点优化DO通过靶向治疗。
Background: Applying mesenchymal stem cells (MSCs), together with the distraction osteogenesis (DO) process, displayed enhanced bone quality and shorter treatment periods. The DO guides the differentiation of MSCs by providing mechanical clues. However, the underlying key genes and pathways are largely unknown. The aim of this study was to screen and identify hub genes involved in distraction-induced osteogenesis of MSCs and potential molecular mechanisms. Material and Methods: The datasets were downloaded from the ArrayExpress database. Three samples of negative control and two samples subjected to 5% cyclic sinusoidal distraction at 0.25 Hz for 6 h were selected for screening differentially expressed genes (DEGs) and then analysed via bioinformatics methods. The Gene Ontology (GO) terms and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment were investigated. The protein–protein interaction (PPI) network was visualised through the Cytoscape software. Gene set enrichment analysis (GSEA) was conducted to verify the enrichment of a self-defined osteogenic gene sets collection and identify osteogenic hub genes. Results: Three hub genes (IL6, MMP2, and EP300) that were highly associated with distraction-induced osteogenesis of MSCs were identified via the Venn diagram. These hub genes could provide a new understanding of distraction-induced osteogenic differentiation of MSCs and serve as potential gene targets for optimising DO via targeted therapies.
登录
查看更多内容
影响因子：
14.9
作者：
Jensen LJ;Kuhn M;Stark M;Chaffron S;Creevey C;Muller J;Doerks T;Julien P;Roth A;Simonovic M;Bork P;von Mering C
通讯作者：
von Mering C
影响因子：
0.8
作者：
Gubin AV;Borzunov DY;Marchenkova LO;Malkova TA;Smirnova IL
通讯作者：
Smirnova IL
影响因子：
1.9
作者：
Borzunov, Dmitry Y.;Kolchin, Sergei N.;Malkova, Tatiana A.
通讯作者：
Malkova, Tatiana A.
靶向沉默 miRNA-132-3p 表达可通过促进小鼠间充质干细胞成骨分化和成骨来挽救废用性骨质减少。
影响因子：
7.5
作者：
Hu, Zebing;Zhang, Lijun;Zhang, Ge
通讯作者：
Zhang, Ge
影响因子：
4.2
作者：
Cho, T. -J.;Kim, J. A.;Choi, I. H.
通讯作者：
Choi, I. H.