Clonal Distribution of Invasive Neisseria meningitidis Isolates from the Norwegian County of Telemark, 1987 to 1995
Clonal Distribution of Invasive Neisseria meningitidis Isolates from the Norwegian County of Telemark, 1987 to 1995
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1987 年至 1995 年挪威泰勒马克郡侵袭性脑膜炎奈瑟菌分离株的克隆分布
DOI：
10.1128/jcm.36.9.2623-2628.1998
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发表时间：
1998
影响因子：
9.4
通讯作者：
L. Frøholm
中科院分区：
文献类型：
--
作者：
Randi Kersten Aakre;A. Jenkins;B. Kristiansen;L. Frøholm
从挪威泰勒马克县脑膜炎球菌病患者中分离出42株脑膜炎奈瑟菌（Neisseria meningitidis）（1987年1月至1995年3月），并通过dhps基因的PCR扩增子限制性内切酶分析（PCR-AREA）、染色体DNA指纹分析和血清学分析进行比较。PCR-AREA将分离株分为11类，其中4类（15、8、6和2株）为克隆型，其余8类为遗传异质型或仅含1株分离株。四个克隆类中的三个可以暂时等同于公认的流行克隆（ET 5，ET 37，和集群A4）的基础上，他们的表型特征，而其余的克隆似乎是新的。克隆的地理分布有显着差异，1类（ET 5样）菌株显着过度代表在农村地区的泰勒马克。1类（ET 5样）分离株发生在整个研究期间，并在1987年占主导地位。2类（ET 37样）分离株出现于1988年至1992年，3类分离株（没有可识别的ET亲和力）仅在1991年和1992年发现。
ABSTRACT Forty-two Neisseria meningitidis isolates were obtained from patients with meningococcal disease in the Norwegian county of Telemark (January 1987 to March 1995), and all were compared by PCR amplicon restriction endonuclease analysis (PCR-AREA) of thedhps gene, chromosomal DNA fingerprinting, and serological analysis. PCR-AREA divided the isolates into 11 classes, of which 4, comprising 15, 8, 6, and 2 isolates, were clonal while the remaining 8 classes were genetically heterogeneous or contained only 1 isolate. Three of the four clonal classes could be tentatively equated with recognized epidemic clones (ET5, ET37, and cluster A4) on the basis of their phenotypic characteristics, while the remaining clone appears to be new. There were significant differences in the geographical distribution of clones, with class 1 (ET5-like) isolates significantly overrepresented in rural parts of Telemark. Class 1 (ET5-like) isolates occurred throughout the study period and were dominant in 1987. Class 2 (ET37-like) isolates occurred from 1988 to 1992, and class 3 isolates (with no recognizable ET affinities) were found only in 1991 and 1992.
使用任意引发的聚合酶链反应分析来确定大学暴发期间分离出的脑膜炎奈瑟氏菌的疾病类型和携带菌株。
DOI：
10.1093/infdis/169.6.1384
发表时间：
1994
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Woods,JP;Kersulyte,D;TolanJr,RW;Berg,CM;Berg,DE
通讯作者：
Berg,DE