Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei.
Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei.
复制标题
DOI：
10.1186/1471-2180-14-92
复制
发表时间：
2014-04-14
期刊：
影响因子：
4.2
通讯作者：
Hogan RJ
中科院分区：
文献类型：
--
作者：
Lafontaine ER;Balder R;Michel F;Hogan RJ
自转运蛋白是一个大家族的外膜蛋白，它决定了革兰氏阴性菌的多种生物学特性。在这项研究中，我们报告了一种新的自转运蛋白基因产物的鼻疽伯克霍尔德菌（位点标签BMA 1027菌株ATCC 23344）的鉴定和表征。数据库检索在至少7个B中鉴定出该基因。发现鼻疽分离物和编码的蛋白质具有84%的相同性。编码ATCC 23344菌株基因组中自转运蛋白的基因的失活显著降低了对HEp-2喉细胞和A549 II型肺细胞单层以及正常人支气管上皮（NHBE）培养物的粘附。与这些发现一致，重组E.大肠杆菌细菌对这些细胞类型的粘附增加5-7倍。在29 B的基因组中鉴定了指定自转运蛋白的基因。假鼻疽分离株和菌株DD 503中基因的破坏使对NHBE培养物的粘附降低了61%。不像B。在鼻疽中，该突变不损害B的结合。假鼻疽对A549或HEp-2细胞的作用。通过气溶胶途径感染B的小鼠的血清分析。鼻疽和B.类鼻疽假单胞菌的研究揭示，接种了少至10种生物体的动物产生抗自身转运蛋白的抗体，因此表明在体内表达。我们的数据表明，我们已经确定了一个自转运蛋白共同的致病物种B。鼻疽和B.类鼻疽杆菌，其介导对呼吸道上皮细胞的粘附并在气溶胶感染过程中在体内表达。
Autotransporters form a large family of outer membrane proteins specifying diverse biological traits of Gram-negative bacteria. In this study, we report the identification and characterization of a novel autotransporter gene product of Burkholderia mallei (locus tag BMA1027 in strain ATCC 23344). Database searches identified the gene in at least seven B. mallei isolates and the encoded proteins were found to be 84% identical. Inactivation of the gene encoding the autotransporter in the genome of strain ATCC 23344 substantially reduces adherence to monolayers of HEp-2 laryngeal cells and A549 type II pneumocytes, as well as to cultures of normal human bronchial epithelium (NHBE). Consistent with these findings, expression of the autotransporter on the surface of recombinant E. coli bacteria increases adherence to these cell types by 5–7 fold. The gene specifying the autotransporter was identified in the genome of 29 B. pseudomallei isolates and disruption of the gene in strain DD503 reduced adherence to NHBE cultures by 61%. Unlike B. mallei, the mutation did not impair binding of B. pseudomallei to A549 or HEp-2 cells. Analysis of sera from mice infected via the aerosol route with B. mallei and B. pseudomallei revealed that animals inoculated with as few as 10 organisms produce antibodies against the autotransporter, therefore indicating expression in vivo. Our data demonstrate that we have identified an autotransporter protein common to the pathogenic species B. mallei and B. pseudomallei which mediates adherence to respiratory epithelial cells and is expressed in vivo during the course of aerosol infection.
登录
查看更多内容
影响因子：
3.1
作者：
Bullard, B;Lipski, SL;Lafontaine, ER
通讯作者：
Lafontaine, ER
影响因子：
4.2
作者：
Bullard, Brian;Lipski, Serena;Lafontaine, Eric R.
通讯作者：
Lafontaine, Eric R.
影响因子：
5.2
作者：
Lazar Adler NR;Stevens JM;Stevens MP;Galyov EE
通讯作者：
Galyov EE
影响因子：
5.5
作者：
Ciabattini, Annalisa;Giomarelli, Barbara;Pozzi, Gianni
通讯作者：
Pozzi, Gianni
影响因子：
3.1
作者：
Brooks, Michael J.;Sedillo, Jennifer L.;Gray-Owen, Scott D.
通讯作者：
Gray-Owen, Scott D.