Characteristics of fast mediated bioelectrocatalytic reaction near microelectrodes.
Characteristics of fast mediated bioelectrocatalytic reaction near microelectrodes.
复制标题
微电极附近快速介导的生物电催化反应的特征。
DOI：
10.1039/c4cp00141a
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Kano
中科院分区：
文献类型：
--
作者：
Y. Kitazumi;Tatsuo Noda;O. Shirai;Masahiro Yamamoto;K. Kano
基于理论分析和数值模拟，研究了微电极上酶促反应产生的伪稳态电流。当酶促反应比通过非线性扩散的底物质量传输快得多时，稳态电流与底物浓度成比例。在这样的条件下，反应平面，其中的基质的质量流被转换为介体的，存在于电极表面附近。稳态电流随着衬底扩散系数的增加而增加。相反，介质的扩散系数和浓度对电流的影响较小。这种差异可以根据反作用平面位置的变化来解释。当系统中存在足够量的酶时，该系统可用作电流型生物传感器，其响应与酶活性的任何变化无关。
The pseudo-steady-state current due to a mediated enzymatic reaction on a microelectrode is characterized on the basis of theoretical analysis and numerical simulation. The steady-state current is proportional to substrate concentration when the enzymatic reaction is considerably faster than substrate mass transport via nonlinear diffusion. Under such conditions, the reaction plane, where the mass flow of the substrate is converted to that of the mediator, exists near the electrode surface. The steady-state current increases as the diffusion coefficient of the substrate increases. In contrast, the diffusion coefficient and the concentration of the mediator have minor effects on the current. This difference can be explained on the basis of a change in the reaction plane location. When a sufficient amount of enzyme exists in a system, the system can be used as an amperometric biosensor, the response of which is independent of any change in enzyme activity.
嘉纳健二、辻村圣哉
DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
Kenji Kano;Seiya Tsujimura
通讯作者：
Seiya Tsujimura
影响因子：
4.3
作者：
Tsujimura, Seiya;Kojima, Shinki;Kano, Kenji
通讯作者：
Kano, Kenji