Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming
Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming
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大鼠胎盘脂肪酸增加，但氨基酸和葡萄糖转运蛋白减少可能会改变宫内编程
DOI：
10.1002/jcb.25450
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发表时间：
2016
影响因子：
4
通讯作者：
Nüsken KD
中科院分区：
文献类型：
--
作者：
Nüsken E;Gellhaus A;Kühnel E;Swoboda I;Wohlfarth M;Vohlen C;Schneider H;Dötsch J;Nüsken KD
胎盘营养转运的调节显著影响胎儿发育，并可能改变宫内生长限制（IUGR）和胎儿规划。我们假设胎盘营养转运蛋白受到子宫-胎盘功能不全和产前手术应激的不同影响。妊娠第E19天，采用双侧子宫动静脉结扎术（LIG）、假手术（SOP）或不手术（对照组，C）。术后4 h （LIG, n=20个胎盘；SOP, n=24； C, n=12）、24 h （LIG, n=28； SOP, n=20； C, n=12）、72 h （LIG, n=20； SOP, n=20； C, n=24）行剖宫产取胎盘。采用qRT‐PCR、western blot和免疫组织化学检测胎盘营养转运体脂肪酸（h‐FABP、CD36）、氨基酸（SNAT1、SNAT2）和葡萄糖（GLUT‐1、Connexin 26）的基因和蛋白表达。有趣的是，术后4、24 （SOP显著）和72 h （SOP显著），LIG和SOP动物胎盘中h‐FABP的平均蛋白表达量增加了一倍。72h后CD36蛋白在LIG中显著升高。24 h后，在LIG和SOP中，SNAT1和SNAT2蛋白和基因的表达显著降低。LIG（4小时，72小时）和SOP（24小时）中的GLUT‐1蛋白和LIG（72小时）中的Connexin 26蛋白进一步显著减少。综上所述，胎盘营养转运蛋白受到血流减少和应激的不同影响，可能会改变已经受到干扰的宫内环境，促进IUGR和胎儿规划。脂肪酸运输能力的增加可能影响能量代谢，可能是一种对大脑发育有积极影响的代偿反应。j .细胞。中国生物医学工程学报，2016,31(2):444 - 444。©2015 Wiley期刊公司
Regulation of placental nutrient transport significantly affects fetal development and may modify intrauterine growth restriction (IUGR) and fetal programming. We hypothesized that placental nutrient transporters are differentially affected both by utero‐placental insufficiency and prenatal surgical stress. Pregnant rats underwent bilateral uterine artery and vein ligation (LIG), sham operation (SOP) or no operation (controls, C) on gestational day E19. Placentas were obtained by caesarean section 4 h (LIG, n=20 placentas; SOP, n=24; C, n=12), 24 h (LIG, n=28; SOP, n=20; C, n=12) and 72 h (LIG, n=20; SOP, n=20; C, n=24) after surgery. Gene and protein expression of placental nutrient transporters for fatty acids (h‐FABP, CD36), amino acids (SNAT1, SNAT2) and glucose (GLUT‐1, Connexin 26) were examined by qRT‐PCR, western blot and immunohistochemistry. Interestingly, the mean protein expression of h‐FABP was doubled in placentas of LIG and SOP animals 4, 24 (SOP significant) and 72 h (SOP significant) after surgery. CD36 protein was significantly increased in LIG after 72 h. SNAT1 and SNAT2 protein and gene expressions were significantly reduced in LIG and SOP after 24 h. Further significantly reduced proteins were GLUT‐1 in LIG (4 h, 72 h) and SOP (24 h), and Connexin 26 in LIG (72 h). In conclusion, placental nutrient transporters are differentially affected both by reduced blood flow and stress, probably modifying the already disturbed intrauterine milieu and contributing to IUGR and fetal programming. Increased fatty acid transport capacity may affect energy metabolism and could be a compensatory reaction with positive effects on brain development. J. Cell. Biochem. 117: 1594–1603, 2016. © 2015 Wiley Periodicals, Inc.
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影响因子：
3.8
作者：
D. Novak;M. Beveridge;M. Malandro;J. Seo
通讯作者：
D. Novak;M. Beveridge;M. Malandro;J. Seo
影响因子：
2.5
作者：
AL, MDM;HORNSTRA, G;HUISJES, HJ
通讯作者：
HUISJES, HJ
影响因子：
3.8
作者：
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通讯作者：
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DOI：
10.1152/ajpcell.1996.271.1.c295
发表时间：
1996-07-01
影响因子：
5.5
作者：
Malandro, MS;Beveridge, MJ;Novak, DA
通讯作者：
Novak, DA
DOI：
10.1152/ajpregu.00122.2010
发表时间：
2010-07-01
影响因子：
2.8
作者：
Baserga, Mariana;Kaur, Rajwinderjit;Lane, Robert H.
通讯作者：
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