Suppression of the imprinted gene NNAT and X-chromosome gene activation in isogenic human iPS cells.
Suppression of the imprinted gene NNAT and X-chromosome gene activation in isogenic human iPS cells.
复制标题
DOI：
10.1371/journal.pone.0023436
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Vaziri H
中科院分区：
文献类型：
--
作者：
Teichroeb JH;Betts DH;Vaziri H
Genetic comparison between human embryonic stem cells and induced pluripotent stem cells has been hampered by genetic variation. To solve this problem, we have developed an isogenic system that allows direct comparison of induced pluripotent stem cells (hiPSCs) to their genetically matched human embryonic stem cells (hESCs). We show that hiPSCs have a highly similar transcriptome to hESCs. Global transcriptional profiling identified 102–154 genes (>2 fold) that showed a difference between isogenic hiPSCs and hESCs. A stringent analysis identified NNAT as a key imprinted gene that was dysregulated in hiPSCs. Furthermore, a disproportionate number of X-chromosome localized genes were over-expressed in female hiPSCs. Our results indicate that despite a remarkably close transcriptome to hESCs, isogenic hiPSCs have alterations in imprinting and regulation of X-chromosome genes.
登录
查看更多内容
影响因子：
3.7
作者：
Marchetto MC;Yeo GW;Kainohana O;Marsala M;Gage FH;Muotri AR
通讯作者：
Muotri AR
影响因子：
2.5
作者：
Han, Dong Wook;Im, Young Bin;Lee, Hoon Taek
通讯作者：
Lee, Hoon Taek
影响因子：
4.4
作者：
Evans, HK;Wylie, AA;Jirtle, RL
通讯作者：
Jirtle, RL
影响因子：
4.6
作者：
Guo, Ge;Yang, Jian;Smith, Austin
通讯作者：
Smith, Austin
DOI：
10.1073/pnas.0910012107
发表时间：
2010-03-02
影响因子：
11.1
作者：
Hu, Bao-Yang;Weick, Jason P.;Zhang, Su-Chun
通讯作者：
Zhang, Su-Chun