Type II taste cells participate in mucosal immune surveillance.
Type II taste cells participate in mucosal immune surveillance.
复制标题
DOI：
10.1371/journal.pbio.3001647
复制
发表时间：
2023-01
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
口腔微生物群的多样性和丰富度仅次于肠道微生物群，但其对味觉细胞的影响在很大程度上仍未被探索。使用单细胞 RNASeq，我们发现小鼠味觉细胞，特别是表达味觉 1 受体成员 3 (Tas1r3) 的甜味和鲜味受体细胞，具有让人想起微折叠 (M) 细胞的基因表达特征，微折叠 (M) 细胞是粘膜相关淋巴组织 (MALT)（例如派尔氏淋巴集结和扁桃体中的那些）免疫监视的核心角色。给予肿瘤坏死因子配体超家族成员 11（TNFSF11；也称为 RANKL）（一种 M 细胞分化所需的生长因子）可显着增加 M 细胞增殖以及味觉乳头和野生型 (WT) 小鼠培养的味觉类器官中的标记基因表达。另一方面，缺乏 Spib (SpibKO)（一种 M 细胞发育和再生所需的 RANKL 调节转录因子）的基因敲除小鼠的味觉乳头和类器官未能对 RANKL 做出反应。 SpibKO 小鼠的味觉乳头还表现出 NF-κB 信号通路成分和促炎细胞因子的表达减少，并且吸引的免疫细胞减少。然而，与 WT 小鼠相比，SpibKO 小鼠中脂多糖诱导的细胞因子表达强烈上调。与 M 细胞一样，WT 小鼠的味觉细胞（而非 SpibKO 小鼠）很容易吸收荧光标记的微珠，这是微生物转胞吞作用的代表。 SpibKO 小鼠中味觉细胞亚型的比例没有改变；然而，他们对甜味和鲜味刺激表现出越来越大的吸引力。我们认为味觉细胞参与免疫监视，并可能调整其对微生物信号和感染的味觉反应。
The oral microbiome is second only to its intestinal counterpart in diversity and abundance, but its effects on taste cells remains largely unexplored. Using single-cell RNASeq, we found that mouse taste cells, in particular, sweet and umami receptor cells that express taste 1 receptor member 3 (Tas1r3), have a gene expression signature reminiscent of Microfold (M) cells, a central player in immune surveillance in the mucosa-associated lymphoid tissue (MALT) such as those in the Peyer’s patch and tonsils. Administration of tumor necrosis factor ligand superfamily member 11 (TNFSF11; also known as RANKL), a growth factor required for differentiation of M cells, dramatically increased M cell proliferation and marker gene expression in the taste papillae and in cultured taste organoids from wild-type (WT) mice. Taste papillae and organoids from knockout mice lacking Spib (SpibKO), a RANKL-regulated transcription factor required for M cell development and regeneration on the other hand, failed to respond to RANKL. Taste papillae from SpibKO mice also showed reduced expression of NF-κB signaling pathway components and proinflammatory cytokines and attracted fewer immune cells. However, lipopolysaccharide-induced expression of cytokines was strongly up-regulated in SpibKO mice compared to their WT counterparts. Like M cells, taste cells from WT but not SpibKO mice readily took up fluorescently labeled microbeads, a proxy for microbial transcytosis. The proportion of taste cell subtypes are unaltered in SpibKO mice; however, they displayed increased attraction to sweet and umami taste stimuli. We propose that taste cells are involved in immune surveillance and may tune their taste responses to microbial signaling and infection.
登录
查看更多内容
影响因子：
2.9
作者：
GRAHAM, CS;GRAHAM, BG;SCHIFFMAN, SS
通讯作者：
SCHIFFMAN, SS
味觉的细胞生物学。
影响因子：
7.8
作者：
Chaudhari, Nirupa;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
DOI：
10.1016/j.bbi.2015.04.001
发表时间：
2015-10
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Feng P;Jyotaki M;Kim A;Chai J;Simon N;Zhou M;Bachmanov AA;Huang L;Wang H
通讯作者：
Wang H
影响因子：
5.3
作者：
Feng, Pu;Chai, Jinghua;Wang, Hong
通讯作者：
Wang, Hong
影响因子：
5.3
作者：
de Lau, Wim;Kujala, Pekka;Clevers, Hans
通讯作者：
Clevers, Hans