Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.
Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.
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DOI：
10.1016/j.ajog.2009.07.051
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发表时间：
2010-01
影响因子：
9.8
通讯作者：
Zisch AH
中科院分区：
文献类型：
--
作者：
Bilic G;Brubaker C;Messersmith PB;Mallik AS;Quinn TM;Haller C;Done E;Gucciardo L;Zeisberger SM;Zimmermann R;Deprest J;Zisch AH
本研究旨在测试与胎膜生物相容的可注射外科密封剂，最终用于关闭医源性胎膜缺损。Dermabond、Histoacryl、Tissucol纤维蛋白胶和三种类型的原位形成的聚（乙二醇）基聚合物水凝胶在与胎膜直接接触24小时后进行了急性毒性试验。为了测定洗脱毒性，将密封剂浸提液在羊膜细胞培养物上孵育72小时。通过形态学和/或生物化学分析评估结合和毒性。所有粘合剂的浸提液对培养细胞均无毒。然而，只有Tissucol和一种基于聚（乙二醇）的水凝胶，贻贝模拟组织粘合剂，显示出有效的，非破坏性的，无毒的结合到胎膜。在体外模型中，在用3.5 mm套管针创建的膜缺损上应用仿贻贝组织粘合剂实现了防漏闭合，可承受膜拉伸。一种合成水凝胶型组织粘合剂成为医源性膜缺损的潜在密封方式，值得进一步体内评价。
This study was undertaken to test injectible surgical sealants that are biocompatible with fetal membranes, eventually for closure of iatrogenic membrane defects. Dermabond, Histoacryl, Tissucol fibrin glue, and three types of in situ forming poly(ethylene glycol)-based polymer hydrogels were tested for acute toxicity upon direct contact with fetal membranes for 24h. For determination of elution toxicity, extracts of sealants were incubated on amnion cell cultures for 72h. Bonding and toxicity was assessed through morphological and/or biochemical analysis. Extracts of all adhesives were non-toxic for cultured cells. However, only Tissucol and one type of poly(ethylene glycol)-based hydrogel, mussel-mimetic tissue adhesive, showed efficient, non-disruptive, non-toxic bonding to fetal membranes. Mussel-mimetic tissue adhesive applied over membrane defects created with a 3.5 mm trocar accomplished leak-proof closure that withstood membrane stretch in an in vitro model. A synthetic hydrogel-type tissue adhesive emerged as potential sealing modality for iatrogenic membrane defects that merits further evaluation in vivo.
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影响因子：
2.1
作者：
Quintero, RA
通讯作者：
Quintero, RA
影响因子：
4
作者：
Burke, Sean A.;Ritter-Jones, Marsha;Messersmith, Phillip B.
通讯作者：
Messersmith, Phillip B.
影响因子：
56.9
作者：
Lee, Haeshin;Dellatore, Shara M.;Messersmith, Phillip B.
通讯作者：
Messersmith, Phillip B.
影响因子：
9.8
作者：
Ochsenbein-Koelble, Nicole;Jani, Jacques;Deprest, Jan
通讯作者：
Deprest, Jan
DOI：
10.1073/pnas.91.13.5967
发表时间：
1994-06-21
影响因子：
11.1
作者：
HILLWEST, JL;CHOWDHURY, SM;HUBBELL, JA
通讯作者：
HUBBELL, JA