Morphological analysis of growth hormone release from rat somatotrophs into blood vessels by immunogold electron microscopy.
Morphological analysis of growth hormone release from rat somatotrophs into blood vessels by immunogold electron microscopy.
复制标题
通过免疫金电子显微镜对大鼠生长激素释放到血管中的生长激素进行形态学分析。
作者：
O. Shimada;H. Tosaka
通过免疫金电子显微镜在体内检查大鼠垂体前叶生长激素释放的 GH 超微结构。在戊巴比妥麻醉下，注射 GH 释放因子明显诱导血浆 GH 含量和细胞中胞吐 GH 免疫阳性颗粒数量的增加。胞吐事件发生在面向内分泌细胞（包括其他生长激素细胞）的质膜部分，以及面向滤泡星状细胞或血管壁的其他部分。当从质膜释放时，GH免疫阳性分泌颗粒有时会相互聚集，并呈现出被单个单元膜包围的不规则形状。胞吐分泌颗粒被释放到细胞外空间，然后作为不规则形状的 GH 免疫阳性电子致密团流入血窦。通过内皮细胞之间的细胞间隙到达血管间隙后，每个团块的内容物广泛分散到血流中，同时免疫阳性消失。因此，本研究揭示了生长激素从生长激素分泌到血管的过程的形态学方面。
The ultrastructure of GH release from the somatotrophs of the rat anterior pituitary was examined in vivo by immunogold electron microscopy. Under pentobarbital anesthesia, injection of GH-releasing factor clearly induced an increase in both plasma GH content and the number of exocytotic GH-immunopositive granules in the cells. The exocytotic events occurred from a part of the plasma membrane facing endocrine cells, including other somatotrophs, and other portions facing folliculo-stellate cells or the walls of blood vessels. When released from the plasma membrane, the GH-immunopositive secretory granules sometimes appeared to aggregate with each other and showed an irregular shape surrounded by a single unit membrane. The exocytotic secretory granules were released into the extracellular space, and then flowed into the sinusoids as irregularly shaped GH-immunopositive electron-dense masses. After reaching the vascular space via the intercellular spaces between the endothelial cells, the contents of each mass became diffusely dispersed into the blood stream, with concomitant disappearance of immunopositivity. The present study thus revealed the morphological aspects of the process of GH secretion from somatotrophs into the blood vessels.
生长激素释放因子，生长激素释放因子，刺激麻醉大鼠生长激素的分泌。
DOI：
10.1016/0006-291x(82)91732-6
发表时间：
1982
影响因子：
3.1
作者：
Wehrenberg,WB;Ling,N;Brazeau,P;Esch,F;Böhlen,P;Baird,A;Ying,S;Guillemin,R
通讯作者：
Guillemin,R