Long Non-Coding RNA Expression during Aging in the Human Subependymal Zone.
Long Non-Coding RNA Expression during Aging in the Human Subependymal Zone.
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DOI：
10.3389/fneur.2015.00045
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发表时间：
2015
影响因子：
3.4
通讯作者：
Weickert CS
中科院分区：
文献类型：
--
作者：
Barry G;Guennewig B;Fung S;Kaczorowski D;Weickert CS
人室管膜下区（SEZ）是整个生命中新生神经元的来源，并且神经发生是一个多步骤过程，其需要在细胞增殖和早期神经元成熟期间的不同转录物，沿着细胞状态/命运转变期间基因表达的协调变化。此外，越来越清楚的是，我们基因组中的大多数导致产生非蛋白质编码RNA，在人类大脑的进化，发育，适应和区域特异性功能中起着重要作用。我们预测，在经济特区中表达的一些转录本可能是这个专门的大脑区域所独有的，并且对该区域进行全面的转录组学分析将有助于定义成年人神经元出生和生长过程中的表达变化。在这里，我们在成年和衰老期间使用人类SEZ组织的深度RNA测序来表征转录景观，特别强调长非编码RNA（lncRNA）。这些数据显示了编码增殖、祖细胞和炎症蛋白的mRNA以及在人类SEZ中高度表达的lncRNA的独特子集的预测年龄相关变化，其中许多具有未知功能。我们的研究结果表明，存在强大的增殖和神经元分化的潜力，在成年人的SEZ，并奠定了基础，了解lncRNA参与出生后的神经发生和出生后出现的潜在相关的神经发育疾病。
The human subependymal zone (SEZ) is debatably a source of newly born neurons throughout life and neurogenesis is a multi-step process requiring distinct transcripts during cell proliferation and early neuronal maturation, along with orchestrated changes in gene expression during cell state/fate transitions. Furthermore, it is becoming increasingly clear that the majority of our genome that results in production of non-protein-coding RNAs plays vital roles in the evolution, development, adaptation, and region-specific function of the human brain. We predicted that some transcripts expressed in the SEZ may be unique to this specialized brain region, and that a comprehensive transcriptomic analysis of this region would aid in defining expression changes during neuronal birth and growth in adult humans. Here, we used deep RNA sequencing of human SEZ tissue during adulthood and aging to characterize the transcriptional landscape with a particular emphasis on long non-coding RNAs (lncRNAs). The data show predicted age-related changes in mRNAs encoding proliferation, progenitor, and inflammatory proteins as well as a unique subset of lncRNAs that are highly expressed in the human SEZ, many of which have unknown functions. Our results suggest the existence of robust proliferative and neuronal differentiation potential in the adult human SEZ and lay the foundation for understanding the involvement of lncRNAs in postnatal neurogenesis and potentially associated neurodevelopmental diseases that emerge after birth.
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影响因子：
82.9
作者：
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通讯作者：
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通讯作者：
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