Complex formation between hepatitis C virus core protein and p21Waf1/Cip1/Sdi1.
Complex formation between hepatitis C virus core protein and p21Waf1/Cip1/Sdi1.
复制标题
丙型肝炎病毒核心蛋白与p21Waf1/Cip1/Sdi1之间形成复合物。
DOI：
10.1006/bbrc.2000.2970
复制
发表时间：
2000
影响因子：
3.1
通讯作者：
H. Hotta
中科院分区：
文献类型：
--
作者：
F. Wang;I. Yoshida;M. Takamatsu;S. Ishido;T. Fujita;K. Oka;H. Hotta
丙型肝炎病毒（HCV）核心蛋白（Core）在肝癌发生过程中起重要作用。通过谷胱甘肽S-转移酶（GST）下拉实验，我们发现Core与p21 Waf 1/Cip 1/Sdi 1（p21）细胞周期调节因子形成复合物。缺失图谱分析显示，Core的N端附近的部分（氨基酸24-52）和p21的C端部分（氨基酸139-164）参与复合物的形成。复合物的形成不受p21在残基147、149和150处的点突变的影响，所述点突变已被报道废除p21与增殖细胞核抗原（PCNA）的相互作用，从而将核心结合序列与PCNA结合序列区分开。然而，由于结合位点非常接近，Core和PCNA在与p21相互作用时相互竞争。Core蛋白与p21蛋白的相互作用可能为HCV致病机制的研究提供新的思路。
The core protein (Core) of hepatitis C virus (HCV) has been known to play an important role in hepatocarcinogenesis. By using glutathione S-transferase (GST) pull-down assay, we show here that Core formed a complex with p21Waf1/Cip1/Sdi1 (p21) cell cycle regulator. The deletion-mapping analysis revealed that a portion near the N-terminus of Core (amino acids 24-52) and a C-terminal portion of p21 (amino acids 139-164) were involved in the complex formation. The complex formation was not impaired by point mutations of p21 at residues 147, 149, and 150, which have been reported to abrogate interaction of p21 with proliferating cell nuclear antigen (PCNA), discriminating the Core-binding sequence from the PCNA-binding sequence. Due to the close vicinity of the binding sites, however, Core and PCNA competed with each other when interacting with p21. The distinct interaction between Core and p21 may provide a new aspect to the studies of HCV pathogenesis.
影响因子：
3.7
作者：
NODA, A;NING, Y;SMITH, JR
通讯作者：
SMITH, JR
影响因子：
3.7
作者：
Ray, RB;Meyer, K;Ray, R
通讯作者：
Ray, R
p21(Waf1/Cip1)羧基末端肽在引入人体细胞后表现出细胞周期蛋白依赖性激酶抑制活性和细胞毒性。
影响因子：
11.2
作者：
Mutoh,M;Lung,FD;Long,YQ;Roller,PP;Sikorski,RS;O'Connor,PM
通讯作者：
O'Connor,PM