The Good and the Bad: Ecological Interaction Measurements Between the Urinary Microbiota and Uropathogens.
The Good and the Bad: Ecological Interaction Measurements Between the Urinary Microbiota and Uropathogens.
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DOI：
10.3389/fmicb.2021.659450
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发表时间：
2021
影响因子：
5.2
通讯作者：
de Vos MGJ
中科院分区：
文献类型：
--
作者：
Zandbergen LE;Halverson T;Brons JK;Wolfe AJ;de Vos MGJ
人体在各种生态环境中都有大量的微生物种群。其中一些微生物的生态位，如人类的肠道和呼吸系统，已经得到了很好的研究。研究不足的一个系统是尿路，主要是因为在没有感染的情况下，它被认为是无菌的。多亏了现代测序和增强的培养技术，现在人们知道存在尿液微生物区系。这意味着这些物种以群落的形式生活在尿路中，形成微生物生态系统。然而，在这样的生态系统中，物种之间的相互作用仍然未知。对人体不同部位的各种研究都强调了原有的微生物区系通过直接或间接影响细菌的致病性来改变感染过程的能力。对于尿路，驻留的微生物群对尿路病原体和表型微生物相互作用的影响在很大程度上是未知的。还没有研究测量尿路病原体对常驻尿路细菌的反应。在这项研究中，我们使用条件培养液中的生长测量来研究从患有尿路感染的老年人身上分离的尿路病原体与从无症状个体的尿路中分离的细菌之间的相互作用。我们观察到，从有UTI样症状的个人中分离的细菌和从无症状的个人中分离的细菌会相互影响对方的生长；例如，从有症状的个人中分离的细菌对从无症状的个人中分离的细菌的生长的影响比从无症状的个人中分离的细菌更负面。此外，我们还表明，与革兰氏阴性菌相比，革兰氏阳性菌改变生长特性的方式不同。我们的结果是阐明微生物相互作用在尿路微生物生态系统中的作用的早期步骤，尿路微生物生态系统既有尿路病原体，也有先前存在的微生物区系。
The human body harbors numerous populations of microorganisms in various ecological niches. Some of these microbial niches, such as the human gut and the respiratory system, are well studied. One system that has been understudied is the urinary tract, primarily because it has been considered sterile in the absence of infection. Thanks to modern sequencing and enhanced culture techniques, it is now known that a urinary microbiota exists. The implication is that these species live as communities in the urinary tract, forming microbial ecosystems. However, the interactions between species in such an ecosystem remains unknown. Various studies in different parts of the human body have highlighted the ability of the pre-existing microbiota to alter the course of infection by impacting the pathogenicity of bacteria either directly or indirectly. For the urinary tract, the effect of the resident microbiota on uropathogens and the phenotypic microbial interactions is largely unknown. No studies have yet measured the response of uropathogens to the resident urinary bacteria. In this study, we investigate the interactions between uropathogens, isolated from elderly individuals suffering from UTIs, and bacteria isolated from the urinary tract of asymptomatic individuals using growth measurements in conditioned media. We observed that bacteria isolated from individuals with UTI-like symptoms and bacteria isolated from asymptomatic individuals can affect each other’s growth; for example, bacteria isolated from symptomatic individuals affect the growth of bacteria isolated from asymptomatic individuals more negatively than vice versa. Additionally, we show that Gram-positive bacteria alter the growth characteristics differently compared to Gram-negative bacteria. Our results are an early step in elucidating the role of microbial interactions in urinary microbial ecosystems that harbor both uropathogens and pre-existing microbiota.
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DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Flores-Mireles AL;Walker JN;Caparon M;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
4.3
作者：
Fava, Francesca;Danese, Silvio
通讯作者：
Danese, Silvio
影响因子：
15.5
作者：
Gottschick C;Deng ZL;Vital M;Masur C;Abels C;Pieper DH;Wagner-Döbler I
通讯作者：
Wagner-Döbler I
DOI：
10.1073/pnas.1308523110
发表时间：
2013-08-27
影响因子：
11.1
作者：
Bachmann, Herwig;Fischlechner, Martin;Teusink, Bas
通讯作者：
Teusink, Bas