The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment.
The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment.
复制标题
DOI：
10.1186/s40168-017-0305-3
复制
发表时间：
2017-08-14
期刊：
影响因子：
15.5
通讯作者：
Wagner-Döbler I
中科院分区：
文献类型：
--
作者：
Gottschick C;Deng ZL;Vital M;Masur C;Abels C;Pieper DH;Wagner-Döbler I
泌尿系统微生物群与阴道和阴茎微生物群一样复杂，但其作为病原体和复发性多微生物生物膜疾病（如细菌性阴道病（BV））的储存库的作用尚不清楚。在这里，我们分析了健康男性和女性的尿液微生物群，并使用16S rRNA基因V1-V2区域的下一代测序，将其与女性在BV期间和抗生素治疗后的尿液微生物群进行了比较。在健康人群中鉴定出8种不同的群落类型，即尿型（UT），除UT 7以crispatus乳杆菌相对丰度为主，仅在健康女性中发现外，其余均为男性和女性共有。口服甲硝唑显著降低了香农菌多样性和阴道加德纳菌、阴道托波菌和羊膜Sneathia amnii的平均相对丰度，而L. iners的水平增加到健康女性的两倍。尽管个体尿液微生物谱对抗生素有强烈反应，但健康群体无法恢复。尿液和阴道液微生物群之间的相关性一般较弱，并取决于UT和BV状态。急性细菌性阴道炎中UT - 1最高（59%的样本），但在甲硝唑治疗后，35名妇女中只有3名显示其尿液和阴道微生物群组成之间存在显著相关性。因此，尿道和膀胱中有不同于阴道的微生物群落。男性和女性尿液中高丰度的细菌性阴道炎相关物种表明尿液可能是病原体的储存库，并有助于复发。本文的在线版本（doi:10.1186/s40168-017-0305-3）包含补充材料，可供授权用户使用。
The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear. Here, we analysed the urinary microbiota of healthy men and women and compared it with that of women during BV and after antibiotic treatment using next-generation sequencing of the 16S rRNA gene V1-V2 regions. Eight different community types, so called urotypes (UT), were identified in healthy humans, all of which were shared between men and women, except UT 7, dominated in relative abundance by Lactobacillus crispatus, which was found in healthy women only. Orally applied metronidazole significantly reduced Shannon diversity and the mean relative abundance of Gardnerella vaginalis, Atopobium vaginae, and Sneathia amnii, while L. iners increased to levels twofold higher than those found in healthy women. Although individual urine microbial profiles strongly responded to the antibiotic, the healthy community could not be restored. The correlation between urinary and vaginal fluid microbiota was generally weak and depending on UT and BV status. It was highest in UT 1 in acute BV (59% of samples), but after metronidazole treatment, only 3 out of 35 women showed a significant correlation between their urinary and vaginal microbiota composition. Urethra and bladder thus harbor microbial communities distinct from the vagina. The high abundance of BV related species in the urine of both men and women suggests that urine may act as a reservoir of pathogens and contribute to recurrence. ClinicalTrials.gov, NCT02687789 The online version of this article (doi:10.1186/s40168-017-0305-3) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.7
作者：
Dols JA;Molenaar D;van der Helm JJ;Caspers MP;de Kat Angelino-Bart A;Schuren FH;Speksnijder AG;Westerhoff HV;Richardus JH;Boon ME;Reid G;de Vries HJ;Kort R
通讯作者：
Kort R
影响因子：
4.4
作者：
Fettweis JM;Serrano MG;Sheth NU;Mayer CM;Glascock AL;Brooks JP;Jefferson KK;Vaginal Microbiome Consortium (additional members);Buck GA
通讯作者：
Buck GA
影响因子：
3.7
作者：
Gottschick C;Szafranski SP;Kunze B;Sztajer H;Masur C;Abels C;Wagner-Döbler I
通讯作者：
Wagner-Döbler I
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
5.7
作者：
Lewis DA;Brown R;Williams J;White P;Jacobson SK;Marchesi JR;Drake MJ
通讯作者：
Drake MJ