Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model.
Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model.
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DOI：
10.1111/jam.15812
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发表时间：
2022-12
影响因子：
4
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中科院分区：
文献类型：
--
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使用猪外植体生物膜模型评估了产生H2 O2的电化学绷带（e绷带）对耐甲氧西林金黄色葡萄球菌定植和生物膜去除的影响。评价了e-绷带产生的H2 O2向外植体组织的转运和相关的潜在毒性。在三个离体S.金黄色葡萄球菌感染模型：（1）减少定殖，（2）去除年轻生物膜和（3）去除成熟生物膜。使用微电极测量外植体/生物膜中的H2 O2浓度-深度分布。比较了极化和非极化未感染外植体的真核细胞活力的降低。e-绷带有效地降低了S.金黄色葡萄球菌定殖（p = 0.029），并降低了年轻生物膜的活原核细胞浓度（p = 0.029），对成熟生物膜的影响有限（p > 0.1）。与非极化外植体相比，H2 O2穿透生物膜和外植体并使真核细胞活力降低32-44%。产生H2 O2的电子绷带在用于减少定植和去除年轻生物膜而不是去除成熟生物膜时最活跃。所描述的电子绷带减少了S。aureus定植和年轻S.金黄色葡萄球菌生物膜在猪外植体伤口模型，支持其进一步发展作为一种无抗生素的替代管理生物膜感染。
Effects of H2O2 producing electrochemical‐bandages (e‐bandages) on methicillin‐resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H2O2 produced from the e‐bandage into explant tissue and associated potential toxicity were evaluated. Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e‐bandages in three ex vivo S. aureus infection models: (1) reducing colonization, (2) removing young biofilms and (3) removing mature biofilms. H2O2 concentration‐depth profiles in explants/biofilms were measured using microelectrodes. Reductions in eukaryotic cell viability of polarized and nonpolarized noninfected explants were compared. e‐Bandages effectively reduced S. aureus colonization (p = 0.029) and reduced the viable prokaryotic cell concentrations of young biofilms (p = 0.029) with limited effects on mature biofilms (p > 0.1). H2O2 penetrated biofilms and explants and reduced eukaryotic cell viability by 32–44% compared to nonpolarized explants. H2O2 producing e‐bandages were most active when used to reduce colonization and remove young biofilms rather than to remove mature biofilms. The described e‐bandages reduced S. aureus colonization and young S. aureus biofilms in a porcine explant wound model, supporting their further development as an antibiotic‐free alternative for managing biofilm infections.
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