Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization.
Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization.
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DOI：
10.1371/journal.pone.0247056
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Miyaji EN
中科院分区：
文献类型：
--
作者：
Araujo AP;Colichio GBC;Oliveira MLS;German E;Nikolaou E;Chen T;Adler H;Ferreira DM;Miyaji EN
老年人患肺炎球菌病的风险增加。这项工作的目的是评估是否有任何减少血清IgG抗肺炎球菌表面蛋白A（PspA）和肺炎球菌表面蛋白C（PspC）的抗原的变体在健康成人随着年龄的增长。通过ELISA测定血清样品中抗PspA和PspC变体的IgG水平，将18-30岁的志愿者与50-70+的志愿者在实验性肺炎球菌定殖攻击之前和之后进行比较。攻毒中使用的血清型6 B菌株属于表达两种PspC变体的肺炎球菌分离株的一小部分。对于最常见的PspA变体和所分析的所有PspC变体，IgG水平随着年龄的增加而降低。未发现基础水平的IgG对这些抗原和保护殖民。在定植的年轻个体中，针对PspA变体的IgG水平增加，这些变体与挑战后由挑战菌株表达的变体更具交叉反应性。由于我们研究中使用的攻毒株表达两种不同的PspC变体，因此在定植的年轻个体中观察到针对所有测试的PspC变体的血清IgG增加。对于检测的一些抗原变体，在接受挑战但未定植的年轻志愿者中观察到血清IgG降低。因此，在健康成年人中，针对PspA和PspC变体的血清IgG抗体随着年龄的增长而降低，但针对这些抗原的IgG水平与针对人类实验性定殖的保护之间没有相关性。虽然没有观察到针对PspA和PspC的天然诱导的血清IgG抗体与针对定殖的保护之间的相关性，但是这些结果并不排除这些抗原作为针对肺炎球菌感染的疫苗的保护潜力。
Older adults are at increased risk of pneumococcal disease. This work aims to evaluate whether there is any decrease in serum IgG against variants of the antigens Pneumococcal surface protein A (PspA) and Pneumococcal surface protein C (PspC) in healthy adults with increasing age. Levels of IgG against PspA and PspC variants were determined by ELISA in serum samples comparing volunteers 18–30 years of age with volunteers who were 50–70+ before and after an experimental pneumococcal colonization challenge. The serotype 6B strain used in the challenge belongs to a minor group of pneumococcal isolates expressing two PspC variants. There was a decrease in levels of IgG with increasing age for the most common PspA variants and for all PspC variants analyzed. No correlation was found between basal levels of IgG against these antigens and protection against colonization. There was an increase in levels of IgG against PspA variants that are more cross-reactive with the variant expressed by the challenge strain post challenge in younger individuals who became colonized. Since the challenge strain used in our study expresses two different PspC variants, an increase in serum IgG against all PspC variants tested was observed in younger individuals who became colonized. For some of the antigen variants tested, a decrease in serum IgG was observed in young volunteers who were challenged but did not become colonized. Serum IgG antibodies against PspA and PspC variants thus decrease with age in healthy adults, but there is no correlation between levels of IgG against these antigens and protection against human experimental colonization. Though no correlation between naturally induced serum IgG antibodies against PspA and PspC and protection against colonization was observed, these results do not rule out the protective potential of these antigens as vaccines against pneumococcal infections.
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影响因子：
16.6
作者：
Pathak A;Bergstrand J;Sender V;Spelmink L;Aschtgen MS;Muschiol S;Widengren J;Henriques-Normark B
通讯作者：
Henriques-Normark B
影响因子：
3.6
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de Lencastre, H
影响因子：
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通讯作者：
Hollingshead, SK
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作者：
Moreno, Adriana T.;Oliveira, Maria Leonor S.;Miyaji, Eliane N.
通讯作者：
Miyaji, Eliane N.
影响因子：
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作者：
Ferreira, D. M.;Darrieux, M.;Oliveira, M. L. S.
通讯作者：
Oliveira, M. L. S.