Nitrate Is an Environmental Cue in the Gut for Salmonella enterica Serovar Typhimurium Biofilm Dispersal through Curli Repression and Flagellum Activation via Cyclic-di-GMP Signaling.
Nitrate Is an Environmental Cue in the Gut for Salmonella enterica Serovar Typhimurium Biofilm Dispersal through Curli Repression and Flagellum Activation via Cyclic-di-GMP Signaling.
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DOI：
10.1128/mbio.02886-21
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发表时间：
2021-02-22
期刊：
影响因子：
6.4
通讯作者：
Tükel Ç
中科院分区：
文献类型：
--
作者：
Miller AL;Nicastro LK;Bessho S;Grando K;White AP;Zhang Y;Queisser G;Buttaro BA;Tükel Ç
Curli是肠道细菌生物膜的主要成分，在感染沙门氏菌鼠伤寒血清型后激活模式识别受体并引发关节炎症。的因素，使S。在急性炎症期间，鼠伤寒沙门氏菌从生物膜中分散并侵入上皮以建立成功的感染仍然未知。在这里，我们研究了S。体外和体内的鼠伤寒杆菌生物膜，以了解炎症环境如何调节多细胞和能动S。肠内有鼠伤寒。我们发现宿主产生的硝酸盐是诱导S.鼠伤寒杆菌从生物膜中扩散。硝酸盐抑制生产的一个重要的生物膜组件，卷曲，并通过细胞内的cyclic-di-GMP水平的调制激活鞭毛。我们的结论是，硝酸盐在病原体健身中起着核心作用，通过调节感染过程中的无柄到能动的生活方式转换。
Curli, a major component of the bacterial biofilms in the intestinal tract, activates pattern recognition receptors and triggers joint inflammation after infection with Salmonella enterica serovar Typhimurium. The factors that allow S. Typhimurium to disperse from biofilms and invade the epithelium to establish a successful infection during acute inflammation remain unknown. Here, we studied S. Typhimurium biofilms in vitro and in vivo to understand how the inflammatory environment regulates the switch between multicellular and motile S. Typhimurium in the gut. We discovered that nitrate generated by the host is an environmental cue that induces S. Typhimurium to disperse from the biofilm. Nitrate represses production of an important biofilm component, curli, and activates flagella via the modulation of intracellular cyclic-di-GMP levels. We conclude that nitrate plays a central role in pathogen fitness by regulating the sessile-to-motile lifestyle switch during infection.
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影响因子：
3.2
作者：
Malhotra K;Hunter T;Henry B;Ishmail Y;Gaddameedi P;Tursi S;Tükel Ç;Hoffer M;Buttaro BA;Queisser G
通讯作者：
Queisser G
影响因子：
3.1
作者：
MacKenzie, Keith D.;Wang, Yejun;White, Aaron P.
通讯作者：
White, Aaron P.
影响因子：
3.2
作者：
COLLINSON, SK;EMODY, L;KAY, WW
通讯作者：
KAY, WW
影响因子：
30.3
作者：
Lupp, Claudia;Robertson, Marilyn L.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett
影响因子：
3.1
作者：
Barthel, M;Hapfelmeier, S;Hardt, WD
通讯作者：
Hardt, WD