21-nt phasiRNAs direct target mRNA cleavage in rice male germ cells.
21-nt phasiRNAs direct target mRNA cleavage in rice male germ cells.
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DOI：
10.1038/s41467-020-19034-y
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发表时间：
2020-10-15
影响因子：
16.6
通讯作者：
Qi Y
中科院分区：
文献类型：
--
作者：
Jiang P;Lian B;Liu C;Fu Z;Shen Y;Cheng Z;Qi Y
在禾本科植物中，定相小干扰RNA（phasiRNA），长度为21或24个核苷酸（nt），主要在花药中表达，并在调节雄性育性中起作用。然而，它们的目标和对目标的作用方式仍然未知。在这里，我们分析phasiRNA在水稻减数分裂前和减数分裂小穗以及在纯化的雄性减数分裂前期早期，四分体和小孢子中的表达。我们发现，21-nt的phasiRNA是最丰富的在早期前期的性母细胞，而24-nt的phasiRNA是更丰富的四分体和小孢子。通过进行高灵敏度的degradome测序，我们发现21-nt phasiRNA在雄性生殖细胞中指导靶mRNA切割，特别是在早期前期I的性母细胞中。这些目标包括435个蛋白质编码基因和71个转座子，显示出对碳水化合物生物合成和代谢途径的富集。我们的研究提供了强有力的证据表明，21-nt phasiRNA在靶切割模式中起作用，并且可以通过微调雄性生殖细胞中的碳水化合物生物合成和代谢来促进减数分裂的进展。定相小干扰RNA（Phased small interfering RNA，phasiRNA）在禾本科植物的花药中大量表达。在这里，作者描述了21-nt和24-nt phasiRNA在水稻性母细胞发育的不同阶段的表达，并提供了21-nt phasiRNA指导数百种靶mRNA切割的证据。
In grasses, phased small interfering RNAs (phasiRNAs), 21- or 24-nucleotide (nt) in length, are predominantly expressed in anthers and play a role in regulating male fertility. However, their targets and mode of action on the targets remain unknown. Here we profile phasiRNA expression in premeiotic and meiotic spikelets as well as in purified male meiocytes at early prophase I, tetrads and microspores in rice. We show that 21-nt phasiRNAs are most abundant in meiocytes at early prophase I while 24-nt phasiRNAs are more abundant in tetrads and microspores. By performing highly sensitive degradome sequencing, we find that 21-nt phasiRNAs direct target mRNA cleavage in male germ cells, especially in meiocytes at early prophase I. These targets include 435 protein-coding genes and 71 transposons that show an enrichment for carbohydrate biosynthetic and metabolic pathways. Our study provides strong evidence that 21-nt phasiRNAs act in a target-cleavage mode and may facilitate the progression of meiosis by fine-tuning carbohydrate biosynthesis and metabolism in male germ cells. Phased small interfering RNA (phasiRNAs) are abundantly expressed in the anthers of grasses. Here, the authors profile 21-nt and 24-nt phasiRNA expression at different stages of meiocyte development in rice and provide evidence that 21-nt phasiRNAs direct cleavage of hundreds of target mRNAs.
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影响因子：
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