Unique Features of Mycobacterium abscessus Biofilms Formed in Synthetic Cystic Fibrosis Medium.
Unique Features of Mycobacterium abscessus Biofilms Formed in Synthetic Cystic Fibrosis Medium.
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DOI：
10.3389/fmicb.2021.743126
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发表时间：
2021
影响因子：
5.2
通讯作者：
Jackson M
中科院分区：
文献类型：
--
作者：
Belardinelli JM;Li W;Avanzi C;Angala SK;Lian E;Wiersma CJ;Palčeková Z;Martin KH;Angala B;de Moura VCN;Kerns C;Jones V;Gonzalez-Juarrero M;Davidson RM;Nick JA;Borlee BR;Jackson M
在宿主相关的条件下表征脓肿分枝杆菌复合体(MABSC)生物膜的特征对于针对这种持久的、耐药的胞外杆菌群体设计知情的治疗策略至关重要。使用合成囊性纤维化介质(SCFM)和一种新的生物膜形成模型，我们证明了在这些条件下形成的MABSC生物膜在组成、基因表达谱和应激反应方面与以前报道的在标准实验室介质中生长的生物膜有很大不同。细胞外DNA(EDNA)、含有甘露糖和葡萄糖的多糖和磷脂，而不是蛋白质和真菌酸，被发现是将细菌聚集在一起的关键细胞外基质(ECM)成分。以前报道的影响生物膜发育的环境线索对SCFM生长的生物膜没有任何显著影响，这很可能反映了SCFM是一个营养丰富的环境，MABSC在其中找到了各种应对压力的方法。最后，分子决定因素被确定为可能代表有吸引力的新靶点，以开发针对CF患者MABSC生物膜的辅助治疗。
Characterizing Mycobacterium abscessus complex (MABSC) biofilms under host-relevant conditions is essential to the design of informed therapeutic strategies targeted to this persistent, drug-tolerant, population of extracellular bacilli. Using synthetic cystic fibrosis medium (SCFM) which we previously reported to closely mimic the conditions encountered by MABSC in actual cystic fibrosis (CF) sputum and a new model of biofilm formation, we show that MABSC biofilms formed under these conditions are substantially different from previously reported biofilms grown in standard laboratory media in terms of their composition, gene expression profile and stress response. Extracellular DNA (eDNA), mannose-and glucose-containing glycans and phospholipids, rather than proteins and mycolic acids, were revealed as key extracellular matrix (ECM) constituents holding clusters of bacilli together. None of the environmental cues previously reported to impact biofilm development had any significant effect on SCFM-grown biofilms, most likely reflecting the fact that SCFM is a nutrient-rich environment in which MABSC finds a variety of ways of coping with stresses. Finally, molecular determinants were identified that may represent attractive new targets for the development of adjunct therapeutics targeting MABSC biofilms in persons with CF.
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影响因子：
3
作者：
Carter, G;Wu, M;Bermudez, LE
通讯作者：
Bermudez, LE
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.8
作者：
Bhatt K;Machado H;Osório NS;Sousa J;Cardoso F;Magalhães C;Chen B;Chen M;Kim J;Singh A;Ferreira CM;Castro AG;Torrado E;Jacobs WR Jr;Bhatt A;Saraiva M
通讯作者：
Saraiva M
DOI：
10.1073/pnas.1003219107
发表时间：
2010-07-06
影响因子：
11.1
作者：
Dhar, Neeraj;McKinney, John D.
通讯作者：
McKinney, John D.