Involvement of Met and Kr-h1 in JH-Mediated Reproduction of Female Bactrocera dorsalis (Hendel).
Involvement of Met and Kr-h1 in JH-Mediated Reproduction of Female Bactrocera dorsalis (Hendel).
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Met 和 Kr-h1 参与 JH 介导的雌性橘小实蝇 (Hendel) 繁殖
DOI：
10.3389/fphys.2018.00482
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发表时间：
2018
影响因子：
4
通讯作者：
Dou W
中科院分区：
文献类型：
--
作者：
Yue Y;Yang RL;Wang WP;Zhou QH;Chen EH;Yuan GR;Wang JJ;Dou W
幼虫激素（JH）在昆虫幼虫阶段防止变态和促进成虫的生殖过程。kr<s:1> ppel-homolog 1 （Kr-h1）是一种锌指转录因子，被认为是JH通过JH受体methoprenee - resistant （Met）诱导产生的，可介导JH在昆虫体内的抗变质作用，但其在JH介导的生殖过程中的功能尚未被充分探索。本研究首次从危害果蔬的重要害虫东方果蝇小实蝇（Bactrocera dorsalis）中克隆并鉴定出参与JH信号通路的Met和Kr-h1。随后的时空表达分析显示，Met和Kr-h1分别在7日龄成虫和雌性成虫的脂肪体中高表达。用JH类似物（甲基戊二烯）处理可显著诱导JH信号和卵黄蛋白原（Vg）基因的表达，并加速卵巢发育。RNA干扰（RNAi）进一步发现，在成虫阶段Met或Kr-h1的缺失会阻碍卵巢发育，并显著降低卵子产量。此外，RNAi刺激JH信号和Vg基因表达后，应用甲氧丁烯进行救援。尽管获救昆虫的卵巢表型与rnai前对照仍存在差异，但观察到卵巢重建面积更大，与原始甲基戊二烯实验记录的时空表达和表型一致。我们的数据揭示了Met和Kr-h1参与昆虫卵黄形成和产卵，从而表明JH信号通路在昆虫繁殖中的关键作用。
Juvenile hormone (JH) prevents metamorphosis during insect larval stages and promotes adult reproductive processes. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor assumed to be induced by JH via the JH receptor methoprene-tolerant (Met), mediates the antimetamorphic effect of JH in insects, but its function in JH-mediated reproductive processes has not been fully explored. In this study, Met and Kr-h1 involved in the JH signaling pathway were first cloned and identified from the oriental fruit fly, Bactrocera dorsalis, an important pest infesting fruit and vegetables worldwide. Subsequent spatiotemporal expression analysis revealed that Met and Kr-h1 were both highly expressed in 7-day-old adults and fat body of female adults, respectively. Treatment with a JH analog (methoprene) significantly induced the expression of JH signaling and vitellogenin (Vg) genes and accelerated ovary development. RNA interference (RNAi) further revealed that either Met or Kr-h1 depletion at the adult stage of B. dorsalis impeded ovary development, with significantly lower egg production noted as well. In addition, rescue through methoprene application after RNAi stimulated the expression of JH signaling and Vg genes. Although there were still differences in ovary phenotype between rescued insects and the pre-RNAi control, ovary redevelopment with a larger surface area was observed, consistent with the spatiotemporal expression and phenotypes recorded in the original methoprene experiment. Our data reveal the involvement of Met and Kr-h1 in insect vitellogenesis and egg production, thus indicating the crucial role of the JH signaling pathway in insect reproduction.
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褐飞虱卵巢发育和卵成熟需要耐甲氧普林 (Met) 和 Krupple 同源物 1 (Kr-h1)
影响因子：
4.6
作者：
Lin X;Yao Y;Wang B
通讯作者：
Wang B
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
4.1
作者：
Jin, Tao;Zeng, Ling;Liang, Guangwen
通讯作者：
Liang, Guangwen
影响因子：
3.5
作者：
Chen, L;Zhu, J;Raikhel, AS
通讯作者：
Raikhel, AS
影响因子：
3.8
作者：
Raikhel, AS;Kokoza, VA;Attardo, G
通讯作者：
Attardo, G