Integrated Bioinformatics Analysis and Verification of Gene Targets for Myocardial Ischemia-Reperfusion Injury.
Integrated Bioinformatics Analysis and Verification of Gene Targets for Myocardial Ischemia-Reperfusion Injury.
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DOI：
10.1155/2022/2056630
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发表时间：
2022
影响因子：
--
通讯作者：
Chen, Jian
中科院分区：
文献类型：
--
作者：
Wang, Jianru;Li, Xiaohui;Peng, Guangcao;Fan, Genhao;Zhang, Mengmeng;Chen, Jian
心肌缺血再灌注损伤（MIRI）已成为一个棘手的临床难题。MIRI的病理机制复杂且不明确，因此探索潜在的中枢基因，并通过靶向中枢基因寻找一些对MIRI具有潜在治疗功效的天然产物具有重要意义。首先，通过鲁棒秩聚集算法筛选GSE58486、GSE108940和GSE115568的差异表达基因（DEGs）并进行整合。然后，通过MIRI小鼠的功能实验对枢纽基因进行鉴定和验证。最后，检索到具有MIRI保护作用的天然产物，并进行枢纽基因与天然产物的分子对接模拟。共鉴定出230个整合基因和9个枢纽基因。经验证，Emr1、Tyrobp、Itgb2、Fcgr2b、Cybb、Fcer1g可能是MIRI中最显著的基因。共发现了75种天然产物。其中以二甲苷C、甘草酸、麦冬苷D、多叶根素I、槟榔苷的结合能力较好。Emr1、Tyrobp、Itgb2、Fcgr2b、Cybb和Fcer1g可能在MIRI的病理过程中起关键作用，靶向这些枢纽基因的天然产物（槟榔苷C、甘草酸、麦冬苷D、多叶根素I和槟榔苷）对MIRI显示出潜在的治疗效果。我们的研究结果为探索MIRI的机制和治疗方法提供了新的见解，并揭示了具有MIRI保护作用的天然产物的新的治疗靶点。
Myocardial ischemia-reperfusion injury (MIRI) has become a thorny and unsolved clinical problem. The pathological mechanisms of MIRI are intricate and unclear, so it is of great significance to explore potential hub genes and search for some natural products that exhibit potential therapeutic efficacy on MIRI via targeting the hub genes. First, the differential expression genes (DEGs) from GSE58486, GSE108940, and GSE115568 were screened and integrated via a robust rank aggregation algorithm. Then, the hub genes were identified and verified by the functional experiment of the MIRI mice. Finally, natural products with protective effects against MIRI were retrieved, and molecular docking simulations between hub genes and natural products were performed. 230 integrated DEGs and 9 hub genes were identified. After verification, Emr1, Tyrobp, Itgb2, Fcgr2b, Cybb, and Fcer1g might be the most significant genes during MIRI. A total of 75 natural products were discovered. Most of them (especially araloside C, glycyrrhizic acid, ophiopogonin D, polyphyllin I, and punicalagin) showed good ability to bind the hub genes. Emr1, Tyrobp, Itgb2, Fcgr2b, Cybb, and Fcer1g might be critical in the pathological process of MIRI, and the natural products (araloside C, glycyrrhizic acid, ophiopogonin D, polyphyllin I, and punicalagin) targeting these hub genes exhibited potential therapeutic efficacy on MIRI. Our findings provided new insights to explore the mechanism and treatments for MIRI and revealed new therapeutic targets for natural products with protective properties against MIRI.
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影响因子：
9.3
作者：
Algoet, Michiel;Janssens, Stefan;Oosterlinck, Wouter
通讯作者：
Oosterlinck, Wouter
影响因子：
--
作者：
Li X;Ma N;Xu J;Zhang Y;Yang P;Su X;Xing Y;An N;Yang F;Zhang G;Zhang L;Xing Y
通讯作者：
Xing Y
影响因子：
11.1
作者：
O'Shea KM;Ananthakrishnan R;Li Q;Quadri N;Thiagarajan D;Sreejit G;Wang L;Zirpoli H;Aranda JF;Alberts AS;Schmidt AM;Ramasamy R
通讯作者：
Ramasamy R
影响因子：
3
作者：
Bader, GD;Hogue, CW
通讯作者：
Hogue, CW
DOI：
10.1007/978-1-60761-987-1_18
发表时间：
2011-01-01
期刊：
DATA MINING IN PROTEOMICS: FROM STANDARDS TO APPLICATIONS
影响因子：
--
作者：
Kohl, Michael;Wiese, Sebastian;Warscheid, Bettina
通讯作者：
Warscheid, Bettina