Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters.
Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters.
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DOI：
10.1016/j.ajpath.2021.10.009
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Johns Hopkins COVID-19 Hamster Study Group
中科院分区：
文献类型：
--
作者：
Mulka KR;Beck SE;Solis CV;Johanson AL;Queen SE;McCarron ME;Richardson MR;Zhou R;Marinho P;Jedlicka A;Guerrero-Martin S;Shirk EN;Braxton AM;Brockhurst J;Creisher PS;Dhakal S;Brayton CF;Veenhuis RT;Metcalf Pate KA;Karakousis PC;Zahnow CA;Klein SL;Jain SK;Tarwater PM;Pekosz AS;Villano JS;Mankowski JL;Johns Hopkins COVID-19 Hamster Study Group
为了促进严重急性呼吸综合征冠状病毒2（SARS-CoV-2）研究，包括开发新的干预和预防策略，在一个强大的冠状病毒病2019（COVID-19）动物模型中表征了疾病的进展。在该模型中，雄性和雌性叙利亚金黄仓鼠鼻内接种SARS-CoV-2 USA-WA 1/2020。在接种后2、4、7、14和28天对接种和模拟接种的未感染对照动物组实施安乐死，以跟踪多个临床、病理学、病毒学和免疫学结局。SARS-CoV-2接种动物在感染的第一周内体重持续下降，在终末时间点肺重量较高，并根据组织病理学和定量图像分析测量发生肺实变。在接种后第2天和第4天，呼吸道中可靠地存在高水平的感染性病毒和病毒RNA，对应于广泛的坏死和炎症。在第7天，当感染性病毒的存在是罕见的，间质和肺泡巨噬细胞浸润和显着的修复上皮细胞反应（II型增生）占主导地位的肺。这些病变随时间消退，接种后第28天仅残留上皮修复明显。使用定量方法测量肺中的细胞和形态学改变，为使用仓鼠COVID-19模型开发COVID-19的治疗和预防干预措施提供了有价值的结果指标。
To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2–inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
64.8
作者：
Muñoz-Fontela C;Dowling WE;Funnell SGP;Gsell PS;Riveros-Balta AX;Albrecht RA;Andersen H;Baric RS;Carroll MW;Cavaleri M;Qin C;Crozier I;Dallmeier K;de Waal L;de Wit E;Delang L;Dohm E;Duprex WP;Falzarano D;Finch CL;Frieman MB;Graham BS;Gralinski LE;Guilfoyle K;Haagmans BL;Hamilton GA;Hartman AL;Herfst S;Kaptein SJF;Klimstra WB;Knezevic I;Krause PR;Kuhn JH;Le Grand R;Lewis MG;Liu WC;Maisonnasse P;McElroy AK;Munster V;Oreshkova N;Rasmussen AL;Rocha-Pereira J;Rockx B;Rodríguez E;Rogers TF;Salguero FJ;Schotsaert M;Stittelaar KJ;Thibaut HJ;Tseng CT;Vergara-Alert J;Beer M;Brasel T;Chan JFW;García-Sastre A;Neyts J;Perlman S;Reed DS;Richt JA;Roy CJ;Segalés J;Vasan SS;Henao-Restrepo AM;Barouch DH
通讯作者：
Barouch DH
影响因子：
3.5
作者：
Boesmueller, Hans;Traxler, Selina;Fend, Falko
通讯作者：
Fend, Falko
影响因子：
158.5
作者：
Ackermann, Maximilian;Verleden, Stijn E.;Jonigk, Danny
通讯作者：
Jonigk, Danny
REGN-COV2抗体预防和治疗恒河猕猴和仓鼠中的SARS-COV-2感染。
DOI：
10.1126/science.abe2402
发表时间：
2020-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Baum A;Ajithdoss D;Copin R;Zhou A;Lanza K;Negron N;Ni M;Wei Y;Mohammadi K;Musser B;Atwal GS;Oyejide A;Goez-Gazi Y;Dutton J;Clemmons E;Staples HM;Bartley C;Klaffke B;Alfson K;Gazi M;Gonzalez O;Dick E Jr;Carrion R Jr;Pessaint L;Porto M;Cook A;Brown R;Ali V;Greenhouse J;Taylor T;Andersen H;Lewis MG;Stahl N;Murphy AJ;Yancopoulos GD;Kyratsous CA
通讯作者：
Kyratsous CA