Protein translation and cell death: the role of rare tRNAs in biofilm formation and in activating dormant phage killer genes.
Protein translation and cell death: the role of rare tRNAs in biofilm formation and in activating dormant phage killer genes.
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蛋白质翻译和细胞死亡：稀有TRNA在生物膜形成和激活休眠噬菌体杀手基因中的作用。
DOI：
10.1371/journal.pone.0002394
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发表时间：
2008-06-11
期刊：
影响因子：
3.7
通讯作者：
Wood, Thomas K.
中科院分区：
文献类型：
--
作者：
Garcia-Contreras, Rodolfo;Zhang, Xue-Song;Kim, Younghoon;Wood, Thomas K.
我们以前发现，小大肠杆菌蛋白Hha（溶血素表达调节蛋白）和相邻的，性质不佳的YbaJ是重要的生物膜形成，但是，他们的角色一直模糊。生物膜是复杂的群落，其中细胞信号通常将单细胞转化为原始组织。在这里，我们表明，HHA通过抑制稀有密码子tRNA的转录，从而抑制菌毛的产生，并通过在一定程度上抑制菌毛基因fimA和ihfA的转录，大大减少生物膜的形成。体内结合研究显示Hha与罕见密码子tRNA argU、ileX、ileY和proL以及两个原噬菌体簇D1 P12和CP 4 -57结合。实时PCR证实Hha抑制argU和proL，并且Hha I型菌毛抑制通过添加额外拷贝的argU、ileY和proL而被废除。Hha对稀有密码子tRNA转录的抑制也导致细胞裂解和生物膜分散，这是由于前噬菌体裂解基因rzpD、yfjZ、appY和alpA的激活以及由于ClpP/ClpX蛋白酶的诱导，所述ClpP/ClpX蛋白酶通过降解抗毒素来激活毒素。YbaJ用于介导Hha的毒性。因此，我们已经确定单一蛋白质（Hha）可以通过限制菌毛的产生以及控制细胞死亡来控制生物膜的形成。Hha使用的机制是通过稀有密码子tRNA的可用性来控制翻译，所述稀有密码子tRNA减少菌毛产生并激活原噬菌体裂解基因。因此，Hha与共转录的YbaJ（TomB）一起充当毒素，其减弱Hha毒性。
We discovered previously that the small Escherichia coli proteins Hha (hemolysin expression modulating protein) and the adjacent, poorly-characterized YbaJ are important for biofilm formation; however, their roles have been nebulous. Biofilms are intricate communities in which cell signaling often converts single cells into primitive tissues. Here we show that Hha decreases biofilm formation dramatically by repressing the transcription of rare codon tRNAs which serves to inhibit fimbriae production and by repressing to some extent transcription of fimbrial genes fimA and ihfA. In vivo binding studies show Hha binds to the rare codon tRNAs argU, ileX, ileY, and proL and to two prophage clusters D1P12 and CP4-57. Real-time PCR corroborated that Hha represses argU and proL, and Hha type I fimbriae repression is abolished by the addition of extra copies of argU, ileY, and proL. The repression of transcription of rare codon tRNAs by Hha also leads to cell lysis and biofilm dispersal due to activation of prophage lytic genes rzpD, yfjZ, appY, and alpA and due to induction of ClpP/ClpX proteases which activate toxins by degrading antitoxins. YbaJ serves to mediate the toxicity of Hha. Hence, we have identified that a single protein (Hha) can control biofilm formation by limiting fimbriae production as well as by controlling cell death. The mechanism used by Hha is the control of translation via the availability of rare codon tRNAs which reduces fimbriae production and activates prophage lytic genes. Therefore, Hha acts as a toxin in conjunction with co-transcribed YbaJ (TomB) that attenuates Hha toxicity.
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影响因子：
3.5
作者：
CHEREPANOV, PP;WACKERNAGEL, W
通讯作者：
WACKERNAGEL, W
影响因子：
3.2
作者：
Barrios, AFG;Zuo, RJ;Wood, TK
通讯作者：
Wood, TK
影响因子：
3.2
作者：
Balsalobre, C;Johansson, J;Muñoa, FJ
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Muñoa, FJ
影响因子：
3.2
作者：
Fahlen, TF;Wilson, RL;Jones, BD
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Jones, BD
影响因子：
3.2
作者：
GALLY, DL;RUCKER, TJ;BLOMFIELD, IC
通讯作者：
BLOMFIELD, IC