Engineering Resistance to Bacterial Blight and Bacterial Leaf Streak in Rice.
Engineering Resistance to Bacterial Blight and Bacterial Leaf Streak in Rice.
复制标题
水稻白叶枯病和细菌性叶斑病的工程抗性
DOI：
10.1186/s12284-021-00482-z
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发表时间：
2021-04-23
期刊：
影响因子：
--
通讯作者：
Huang S
中科院分区：
文献类型：
--
作者：
Ni Z;Cao Y;Jin X;Fu Z;Li J;Mo X;He Y;Tang J;Huang S
水稻黄单胞菌（Xanthomonasericum，Xo）是影响水稻生产的重要病原菌群之一。其致病变种为黄单胞菌黄单胞菌致病变种。（Xoo）和黄单胞菌黄单胞菌致病变种（Xanthomonasplantypv.）oryzicola（Xoc）分别引起水稻白叶枯病和细菌性条斑病。Xo主要依靠其转录激活因子样效应子（TALE）与宿主DNA靶标（称为效应子结合元件（EBE））结合并诱导下游主要易感基因的表达来感染宿主植物。阻断TALE与EBE的结合可以提高水稻对相应XO的抗性。我们利用CRISPR/Cas9对水稻品种桂红1号和中花11号中的三个主要易感基因（OsSWEET 11、OsSWEET 14和OsSULTR 3;6）的EBE进行了编辑。这两个品种都在另一个主要易感基因（OsSWEET 13）的EBE中具有天然的一个碱基突变，该突变不是由相应的TALE诱导的。获得了两个未转基因的目标突变系GT 0105（桂红1号）和ZT 0918（中花11号），它们对Xoo和Xoc的抗性显著增强。此外，在模拟田间条件下，GT 0105和ZT 0918的形态和其他农艺性状与野生型基本相同。本研究中，我们首次报道了通过编辑感病基因启动子获得的工程水稻品系对Xoo和Xoc具有抗性，且其原有的农艺性状不受影响。在线版本包含补充材料，可通过10.1186/s12284-021-00482-z获得。
Xanthomonas oryzae (Xo) is one of the important pathogenic bacterial groups affecting rice production. Its pathovars Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Xo infects host plants by relying mainly on its transcription activator-like effectors (TALEs) that bind to host DNA targets, named effector binding elements (EBEs), and induce the expression of downstream major susceptibility genes. Blocking TALE binding to EBE could increase rice resistance to the corresponding Xo. We used CRISPR/Cas9 to edit the EBEs of three major susceptibility genes (OsSWEET11, OsSWEET14 and OsSULTR3;6) in the rice varieties Guihong 1 and Zhonghua 11. Both varieties have a natural one-base mutation in the EBE of another major susceptibility gene (OsSWEET13) which is not induced by the corresponding TALE. Two rice lines GT0105 (from Guihong 1) and ZT0918 (from Zhonghua 11) with target mutations and transgene-free were obtained and showed significantly enhanced resistance to the tested strains of Xoo and Xoc. Furthermore, under simulated field conditions, the morphology and other agronomic traits of GT0105 and ZT0918 were basically the same as those of the wild types. In this study, we first reported that the engineering rice lines obtained by editing the promoters of susceptibility genes are resistant to Xoo and Xoc, and their original agronomic traits are not affected. The online version contains supplementary material available at 10.1186/s12284-021-00482-z.
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影响因子：
6.7
作者：
Tran TT;Pérez-Quintero AL;Wonni I;Carpenter SCD;Yu Y;Wang L;Leach JE;Verdier V;Cunnac S;Bogdanove AJ;Koebnik R;Hutin M;Szurek B
通讯作者：
Szurek B
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
影响因子：
10.5
作者：
Chu, Zhaohui;Yuan, Meng;Wang, Shiping
通讯作者：
Wang, Shiping
影响因子：
7.2
作者：
Hutin, Mathilde;Sabot, Francois;Szurek, Boris
通讯作者：
Szurek, Boris
影响因子：
27.5
作者：
Xu, Zhengyin;Xu, Xiameng;Chen, Gongyou
通讯作者：
Chen, Gongyou