Two-Photon Excited Fluorescence Lifetime Imaging of Tetracycline-Labeled Retinal Calcification.
Two-Photon Excited Fluorescence Lifetime Imaging of Tetracycline-Labeled Retinal Calcification.
复制标题
DOI：
10.3390/s23146626
复制
发表时间：
2023-07-24
期刊：
影响因子：
--
通讯作者：
Thompson RB
中科院分区：
文献类型：
--
作者：
Hegde KR;Ray K;Szmacinski H;Sorto S;Puche AC;Lengyel I;Thompson RB
含钙矿物如羟基磷灰石和白锁石在视网膜视网膜下色素上皮(SubRPE)间隙的沉积与老年性黄斑变性(AMD)的发生和进展有关。在发达国家，AMD是导致老年人失明的最常见的眼病；早期诊断是可取的，特别是在可能的情况下开始治疗。RPE下间隙的钙化也与其他疾病直接相关，如弹性假性黄色瘤(PXE)。我们发现，这些矿藏可以用四环素类抗生素作为特异染料进行荧光成像。四环素与矿物的结合伴随着荧光强度和荧光寿命的增加。四环素的寿命与现有天然视网膜荧光团的已知本底寿命有很大不同，这表明钙化可以通过寿命成像来可视化。然而，用于激发这些寿命变化的激发波长通常比批准的视网膜成像波长要短。在这里，我们展示了人死后视网膜中四环素染色的玻璃体可以通过多光子(红外)激发的荧光寿命对比度来成像。在这项初步研究中，来自六名匿名已故捐赠者(3名女性，3名男性，平均年龄83.7岁，范围79-97岁)的10只眼睛在获得马里兰州解剖委员会的知情同意后获得了伦理监督和机构审查委员会的批准。
Deposition of calcium-containing minerals such as hydroxyapatite and whitlockite in the subretinal pigment epithelial (sub-RPE) space of the retina is linked to the development of and progression to the end-stage of age-related macular degeneration (AMD). AMD is the most common eye disease causing blindness amongst the elderly in developed countries; early diagnosis is desirable, particularly to begin treatment where available. Calcification in the sub-RPE space is also directly linked to other diseases such as Pseudoxanthoma elasticum (PXE). We found that these mineral deposits could be imaged by fluorescence using tetracycline antibiotics as specific stains. Binding of tetracyclines to the minerals was accompanied by increases in fluorescence intensity and fluorescence lifetime. The lifetimes for tetracyclines differed substantially from the known background lifetime of the existing natural retinal fluorophores, suggesting that calcification could be visualized by lifetime imaging. However, the excitation wavelengths used to excite these lifetime changes were generally shorter than those approved for retinal imaging. Here, we show that tetracycline-stained drusen in post mortem human retinas may be imaged by fluorescence lifetime contrast using multiphoton (infrared) excitation. For this pilot study, ten eyes from six anonymous deceased donors (3 female, 3 male, mean age 83.7 years, range 79–97 years) were obtained with informed consent from the Maryland State Anatomy Board with ethical oversight and approval by the Institutional Review Board.
登录
查看更多内容
飞秒激光辅助眼科手术：从激光基本面到临床应用。
影响因子：
3.4
作者：
Yan Q;Han B;Ma ZC
通讯作者：
Ma ZC
影响因子：
10.6
作者：
Gorin MB
通讯作者：
Gorin MB
影响因子：
3.7
作者：
Podgorski K;Terpetschnig E;Klochko OP;Obukhova OM;Haas K
通讯作者：
Haas K
影响因子：
2
作者：
Atmeh, A. R.;Chong, E. Z.;Watson, T. F.
通讯作者：
Watson, T. F.
影响因子：
2.7
作者：
Redford, GI;Clegg, RM
通讯作者：
Clegg, RM