Epidermal growth factor receptor-targeted sonoporation with microbubbles enhances therapeutic efficacy in a squamous cell carcinoma model.
Epidermal growth factor receptor-targeted sonoporation with microbubbles enhances therapeutic efficacy in a squamous cell carcinoma model.
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DOI：
10.1371/journal.pone.0185293
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nishihara T
中科院分区：
文献类型：
--
作者：
Hirabayashi F;Iwanaga K;Okinaga T;Takahashi O;Ariyoshi W;Suzuki R;Sugii M;Maruyama K;Tominaga K;Nishihara T
声孔技术是一种利用超声波作用的药物和基因递送系统，可将正常条件下无法进入细胞的外来分子递送到细胞内。我们之前报道过微泡（MB）声孔作用可以实现对人牙龈鳞状细胞癌 Ca9-22 细胞的有效细胞内药物递送。在这项研究中，我们开发了抗表皮生长因子受体（EGFR）抗体结合的MB（EGFR-MB），并评估了它们在体外和体内增强抗癌药物毒性的能力。我们首先通过 WST-8 测定、流式细胞术和 Hoechst 染色体外评估了 EGFR-MB 声孔作用对 Ca9-22 细胞的影响。声孔法和EGFR-MB对小剂量博来霉素对Ca9-22细胞有很强的细胞毒作用。此外，使用 EGFR-MB 声孔法递送博莱霉素显着增加了凋亡细胞的数量。接下来我们检查了 EGFR-MB 在小鼠鳞状细胞癌模型中的作用。通过 EGFR-MB 声孔传递博来霉素表现出显着的抗肿瘤活性。总之，我们的结果表明 EGFR-MB 和超声治疗提高了细胞内药物摄取的功效和特异性，表明这可能是口腔鳞状细胞癌化疗治疗的一种新型药物靶向方式。
Sonoporation is a drug and gene delivery system using ultrasonication that allows the intracellular delivery of foreign molecules that cannot enter cells under normal conditions. We previously reported that sonoporation with microbubbles (MBs) could achieve effective intracellular drug delivery to human gingival squamous carcinoma Ca9-22 cells. In this study, we developed anti-epidermal growth factor receptor (EGFR) antibody-conjugated MBs (EGFR-MBs) and evaluated their capacity to enhance anti-cancer drug toxicity in vitro and in vivo. We first assessed the effect of sonoporation with EGFR-MBs on Ca9-22 cells by the WST-8 assay, flow cytometry and Hoechst’s staining in vitro. Sonoporation and EGFR-MB had a strong cytotoxic effect on Ca9-22 cells with low-dose bleomycin. Furthermore, bleomycin delivery using sonoporation with EGFR-MBs remarkably increased the number of apoptotic cells. We next examined the effect of EGFR-MBs in a murine squamous cell carcinoma model. Bleomycin delivery by sonoporation with EGFR-MBs exhibited remarkable antitumor activity. Together, our results show that EGFR-MBs and ultrasound treatment increases the efficacy and specificity of intracellular drug uptake, suggesting this could be a novel drug-targeting modality for oral squamous cell carcinoma chemotherapy treatment.
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影响因子：
4.6
作者：
Endo-Takahashi Y;Negishi Y;Nakamura A;Ukai S;Ooaku K;Oda Y;Sugimoto K;Moriyasu F;Takagi N;Suzuki R;Maruyama K;Aramaki Y
通讯作者：
Aramaki Y
DOI：
10.1143/jjap.38.3014
发表时间：
1999-05-01
期刊：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS
影响因子：
--
作者：
Tachibana, K;Tachibana, S
通讯作者：
Tachibana, S
影响因子：
10.8
作者：
Dewitte, Heleen;Van Lint, Sandra;Lentacker, Ine
通讯作者：
Lentacker, Ine
影响因子：
6.2
作者：
Armstrong, Jonathan K.;Hempel, Georg;Garratty, George
通讯作者：
Garratty, George
影响因子：
4.5
作者：
Lamanauskas, N.;Novell, A.;Bouakaz, A.
通讯作者：
Bouakaz, A.