MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii.
MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii.
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DOI：
10.1038/srep38228
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发表时间：
2016-12-02
影响因子：
4.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Gao X;Zhang F;Hu J;Cai W;Shan G;Dai D;Huang K;Wang G
microRNA在高等植物和动物的非生物胁迫反应中发挥重要作用，但其在藻类胁迫适应中的作用尚不清楚。在这项研究中，确定和推定的miRNA在莱茵衣藻的表达进行了评估，使用定量聚合酶链反应;一些miRNA（Cre-miR 906 - 3 p）上调，而其他（Cre-miR 910）下调时，该物种受到多种非生物胁迫。利用degradome测序数据，我们还鉴定了ATP 4（ATP合酶的d-亚基）和NCR 2（NADPH：细胞色素P450还原酶）分别作为Cre-miR 906 - 3 p和Cre-miR 910的几个靶点之一。Q-PCR数据表明，ATP 4在胁迫条件下与Cre-miR 906 - 3 p成反比表达。Cre-miR 906 - 3 p的过表达增强了对多重胁迫的抗性;相反，ATP 4的过表达产生了相反的效果。这些Q-PCR、degradome测序和过表达系的适应性数据表明Cre-miR 906 - 3 p及其靶ATP 4是胁迫适应的同一途径的一部分。我们发现Cre-miR 910及其靶点NCR 2也是该通路的一部分。Cre-miR 910的过表达降低了细胞对多重胁迫的适应能力，而NCR 2的过表达增强了细胞对多重胁迫的适应能力。我们的研究结果表明，这两类miRNA协同介导藻类的胁迫适应。
MicroRNAs play an important role in abiotic stress responses in higher plants and animals, but their role in stress adaptation in algae remains unknown. In this study, the expression of identified and putative miRNAs in Chlamydomonas reinhardtii was assessed using quantitative polymerase chain reaction; some of the miRNAs (Cre-miR906-3p) were up-regulated, whereas others (Cre-miR910) were down-regulated when the species was subjected to multiple abiotic stresses. With degradome sequencing data, we also identified ATP4 (the d-subunit of ATP synthase) and NCR2 (NADPH: cytochrome P450 reductase) as one of the several targets of Cre-miR906-3p and Cre-miR910, respectively. Q-PCR data indicated that ATP4, which was expressed inversely in relation to Cre-miR906-3p under stress conditions. Overexpressing of Cre-miR906-3p enhanced resistance to multiple stresses; conversely, overexpressing of ATP4 produced the opposite effect. These data of Q-PCR, degradome sequencing and adaptation of overexpressing lines indicated that Cre-miR906-3p and its target ATP4 were a part of the same pathway for stress adaptation. We found that Cre-miR910 and its target NCR2 were also a part of this pathway. Overexpressing of Cre-miR910 decreased, whereas that of NCR2 increased the adaption to multiple stresses. Our findings suggest that the two classes of miRNAs synergistically mediate stress adaptation in algae.
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影响因子：
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作者：
Jiang, Wenzhi;Brueggeman, Andrew J.;Weeks, Donald P.
通讯作者：
Weeks, Donald P.
影响因子：
11.4
作者：
Mallory, AC;Reinhart, BJ;Bartel, DP
通讯作者：
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DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
4.7
作者：
Khraiwesh, Basel;Zhu, Jian-Kang;Zhu, Jianhua
通讯作者：
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影响因子：
7.2
作者：
Hu, Jinlu;Deng, Xuan;Huang, Kaiyao
通讯作者：
Huang, Kaiyao
影响因子：
11.6
作者：
Geisler, Daniela A.;Paepke, Carola;Persson, Staffan
通讯作者：
Persson, Staffan