Interleukin-10/Ceftriaxone prevents E. coli-induced delays in sensorimotor task learning and spatial memory in neonatal and adult Sprague-Dawley rats.
Interleukin-10/Ceftriaxone prevents E. coli-induced delays in sensorimotor task learning and spatial memory in neonatal and adult Sprague-Dawley rats.
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DOI：
10.1016/j.brainresbull.2009.10.016
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发表时间：
2010-01-15
影响因子：
3.8
通讯作者：
Bennett WA
中科院分区：
文献类型：
--
作者：
Wallace KL;Lopez J;Shaffery JP;Wells A;Paul IA;Bennett WA
怀孕期间的宫内感染与胎儿免疫系统的早期激活和神经发育不良有关。免疫激活可以导致感觉运动技能的改变、学习和记忆以及神经可塑性的改变。白介素 10 (IL-10) 和头孢曲松均已被证明可以分别降低免疫系统的激活并增加记忆能力。使用宫内感染的啮齿动物模型，我们通过莫里斯水迷宫检查了幼鼠的感觉运动发育、学习和记忆，以及成年大鼠的长期增强。妊娠第 17 天的怀孕大鼠接种 1 × 105 菌落形成单位的大肠杆菌 (E. coli) 或生理盐水。治疗组中的动物在施用大肠杆菌后接受IL-10/头孢曲松3天。宫内感染延迟了表面翻正、负趋地性、惊吓反应和睁眼反应。 IL-10/头孢曲松治疗减少了这些测试的延迟。宫内感染损害了 Morris 水迷宫探针试验的表现（盐水 25.13 ± 1.01；大肠杆菌 20.75 ± 1.01；大肠杆菌 + IL-10/头孢曲松 20.2 ± 1.62），并降低了长时程增强的诱导（盐水 141.5 ± 4.3；大肠杆菌 128.7 ± 3.9；大肠杆菌+ IL-10/头孢曲松 140.0 ± 10)。总之，本研究的结果表明，大肠杆菌诱导的宫内感染会延迟感觉运动以及学习和记忆，而 IL-10/头孢曲松可以挽救其中一些行为。这些延迟还伴随着白细胞介素 1β 水平的增加，这表明免疫激活。 IL-10/头孢曲松可以防止这些延迟，并减少大肠杆菌诱导的白细胞介素 1β 激活，并可能提供一个可以进行适当治疗的时间窗口。
Intrauterine infection during pregnancy is associated with early activation of the fetal immune system and poor neurodevelopmental outcomes. Immune activation can lead to alterations in sensorimotor skills, changes in learning and memory and neural plasticity. Both interleukin-10 (IL-10) and Ceftriaxone have been shown to decrease immune system activation and increase memory capacity, respectively. Using a rodent model of intrauterine infection, we examined sensorimotor development in pups, learning and memory, via the Morris water maze, and long-term potentiation in adult rats. Pregnant rats at gestational day 17 were inoculated with 1 × 105 colony forming units of Escherichia coli (E. coli) or saline. Animals in the treatment group received IL-10/Ceftriaxone for 3 days following E. coli administration. Intrauterine infection delayed surface righting, negative geotaxis, startle response and eye opening. Treatment with IL-10/Ceftriaxone reduced the delay in these tests. Intrauterine infection impaired performance in the probe trial in the Morris water maze (saline 25.13 ± 1.01; E. coli 20.75 ± 1.01; E. coli + IL-10/Ceftriaxone 20.2 ± 1.62) and reduced the induction of long-term potentiation (saline 141.5 ± 4.3; E. coli 128.7 ± 3.9; E. coli + IL-10/Ceftriaxone 140.0 ± 10). In summary, the results of this study indicate that E. coli induced intrauterine infection delays sensorimotor and learning and memory, while IL-10/Ceftriaxone rescues some of these behaviors. These delays were also accompanied by an increase in interleukin-1β levels, which indicates immune activation. IL-10/Ceftriaxone prevents these delays as well as decreases E. coli-induced interleukin-1β activation and may offer a window of time in which suitable treatment could be administered.
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影响因子：
4.2
作者：
Bell, MJ;Hallenbeck, JM
通讯作者：
Hallenbeck, JM
影响因子：
4.2
作者：
Fan, LW;Pang, Y;Cai, ZW
通讯作者：
Cai, ZW
影响因子：
--
作者：
Dubray, Christine;Ibrahim, Salah A.;Brown, Vincent
通讯作者：
Brown, Vincent
影响因子：
15.1
作者：
GIBERTINI, M;NEWTON, C;KLEIN, TW
通讯作者：
KLEIN, TW
影响因子：
3.6
作者：
Cai, ZW;Pan, ZL;Rhodes, PG
通讯作者：
Rhodes, PG