A Distinct Faecal Microbiota and Metabolite Profile Linked to Bowel Habits in Patients with Irritable Bowel Syndrome.
A Distinct Faecal Microbiota and Metabolite Profile Linked to Bowel Habits in Patients with Irritable Bowel Syndrome.
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DOI：
10.3390/cells10061459
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发表时间：
2021-06-10
期刊：
影响因子：
6
通讯作者：
Öhman L
中科院分区：
文献类型：
--
作者：
Ahluwalia B;Iribarren C;Magnusson MK;Sundin J;Clevers E;Savolainen O;Ross AB;Törnblom H;Simrén M;Öhman L
肠易激综合征（IBS）患者的肠道微环境发生改变。因此，我们的目的是确定肠道微环境的概况，基于粪便微生物群和代谢产物，以及与IBS患者症状的潜在联系。通过GA-mapTM微生态失调测试评估粪便微生物群，并使用串联质谱法（GC-MS/MS）对IBS患者和健康受试者的粪便代谢组学进行分析。使用IBS严重程度评分系统评估症状严重程度，使用医院焦虑和抑郁量表评估焦虑和抑郁。基于粪便微生物群（n = 54）和代谢物（n = 155）的主成分分析显示IBS患者（n = 40）和健康受试者（n = 18）之间的明显分离。梅特涅是这种分离的主要驱动力。此外，肠易激综合征患者便秘（n = 15）和腹泻（n = 11）之间的肠道微环境特征不同，而根据症状严重程度或焦虑的患者亚组中未检测到聚类。此外，独创性途径分析预测了IBS患者的氨基酸代谢和几种细胞和分子功能的改变。IBS患者具有与排便习惯相关的独特粪便微生物群和代谢产物谱。基于粪便微生物群和代谢产物的肠道微环境分析可以被认为是未来的非侵入性诊断工具，同时为IBS的病理生理学提供有价值的见解。
Patients with irritable bowel syndrome (IBS) are suggested to have an altered intestinal microenvironment. We therefore aimed to determine the intestinal microenvironment profile, based on faecal microbiota and metabolites, and the potential link to symptoms in IBS patients. The faecal microbiota was evaluated by the GA-mapTM dysbiosis test, and tandem mass spectrometry (GC-MS/MS) was used for faecal metabolomic profiling in patients with IBS and healthy subjects. Symptom severity was assessed using the IBS Severity Scoring System and anxiety and depression were assessed using the Hospital Anxiety and Depression Scale. A principal component analysis based on faecal microbiota (n = 54) and metabolites (n = 155) showed a clear separation between IBS patients (n = 40) and healthy subjects (n = 18). Metabolites were the main driver of this separation. Additionally, the intestinal microenvironment profile differed between IBS patients with constipation (n = 15) and diarrhoea (n = 11), while no clustering was detected in subgroups of patients according to symptom severity or anxiety. Furthermore, ingenuity pathway analysis predicted amino acid metabolism and several cellular and molecular functions to be altered in IBS patients. Patients with IBS have a distinct faecal microbiota and metabolite profile linked to bowel habits. Intestinal microenvironment profiling, based on faecal microbiota and metabolites, may be considered as a future non-invasive diagnostic tool, alongside providing valuable insights into the pathophysiology of IBS.
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