An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
An investigation of fecal volatile organic metabolites in irritable bowel syndrome.
复制标题
肠易激综合征中粪便挥发性有机代谢产物的研究。
DOI：
10.1371/journal.pone.0058204
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Probert CS
中科院分区：
文献类型：
--
作者：
Ahmed I;Greenwood R;Costello Bde L;Ratcliffe NM;Probert CS
诊断肠易激综合征（IBS）可能是一个挑战；许多临床医生采取侵入性检查，以排除其他疾病并使患者放心。挥发性有机代谢物（VOMs）从粪便中排放；了解这些VOMs模式的变化可以帮助我们了解疾病的病因和生物标志物的发展，这有助于IBS的诊断。我们首次报道了腹泻型肠易激综合征（IBS- d）、活动性克罗恩病（CD）、溃疡性结肠炎（UC）和健康对照患者粪便VOMs模式的综合研究。30例IBS-D， 62例CD， 48例UC和109例健康对照。使用Manning标准诊断IBS-D，所有CD和UC患者均符合内窥镜、组织学和/或放射学标准。采用固相微萃取法（SPME）提取粪便VOMs，气相色谱-质谱联用（GC-MS）分析。共鉴定出240个vom。单因素分析显示，短链脂肪酸酯类、环己烷羧酸及其酯类衍生物与IBD相关（p<0.05），醛类在IBD中含量较高（p<0.05）。通过多变量分析建立的预测模型将IBS-D与活动性CD、UC和健康对照分开，灵敏度分别为94%、96%和90%；特异性分别为82%、80%和80% （p<0.05）。了解这些VOMs的来源可能有助于了解IBS-D和IBD的病因学。这些数据表明，粪便VOMs分析可能有助于IBS-D的诊断，这是没有实验室测试的，以及IBD。
Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p<0.05), while aldehydes were more abundant in IBD (p<0.05). A predictive model, developed by multivariate analysis, separated IBS-D from active CD, UC and healthy controls with a sensitivity of 94%, 96% and 90%; and a specificity of 82%, 80% and 80% respectively (p<0.05). The understanding of the derivation of these VOMs may cast light on the etiology of IBS-D and IBD. These data show that fecal VOMs analyses could contribute to the diagnosis of IBS-D, for which there is no laboratory test, as well as IBD.
登录
查看更多内容
影响因子：
8
作者：
Garcia-Sanchez, Valle;Iglesias-Flores, Eva;Gomez-Camacho, Federico
通讯作者：
Gomez-Camacho, Federico
影响因子：
5.2
作者：
De Preter, Vicky;Ghebretinsae, Aklilu Habteab;Verbeke, Kristin
通讯作者：
Verbeke, Kristin
DOI：
10.1016/j.trstmh.2009.02.004
发表时间：
2009-11-01
影响因子：
2.2
作者：
Garner, C. E.;Smith, S.;Probert, C. S. J.
通讯作者：
Probert, C. S. J.
影响因子：
3.8
作者：
Costello, B. De Lacy;Ewen, R.;Smith, S.
通讯作者：
Smith, S.
影响因子：
3.7
作者：
Abraham, Bincy P.;Kane, Sunanda
通讯作者：
Kane, Sunanda