Extracellular Vesicles Derived From Talaromyces marneffei Yeasts Mediate Inflammatory Response in Macrophage Cells by Bioactive Protein Components.
Extracellular Vesicles Derived From Talaromyces marneffei Yeasts Mediate Inflammatory Response in Macrophage Cells by Bioactive Protein Components.
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马尔尼菲踝节酵母衍生的细胞外囊泡通过生物活性蛋白成分介导巨噬细胞的炎症反应
DOI：
10.3389/fmicb.2020.603183
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Yang B;Wang J;Jiang H;Lin H;Ou Z;Ullah A;Hua Y;Chen J;Lin X;Hu X;Zheng L;Wang Q
装载有蛋白质、核酸、膜脂和其他毒力因子的细胞外囊泡（EV）可能参与某些真菌（例如新型隐球菌和白色念珠菌）的致病过程。然而，源自马尔尼菲Talaromyces marneffii (TM)的电动汽车的具体特征尚未弄清楚。在本研究中，通过纳米颗粒跟踪分析和透射电子显微镜观察发现，TM 衍生的 EV 是一组异质的纳米尺寸膜囊泡（30-300 nm）。 DiI 标记的 EV 可以被 RAW 264.7 巨噬细胞吸收。 EV与巨噬细胞一起孵育会导致活性氧、一氧化氮和一些炎症因子（包括白细胞介素1β、白细胞介素6、白细胞介素10和肿瘤坏死因子）的表达水平增加。此外，EV刺激的巨噬细胞中共刺激分子（CD80、CD86和MHC-II）的表达也增加。当EV被蛋白酶水解时，巨噬细胞分泌的炎症因子水平显着降低，而DNA和RNA水解酶处理时则保持不变。随后，通过液相色谱-串联质谱法测定了EV中的一些毒力因子，包括热休克蛋白、甘露糖蛋白1和过氧化物酶。综上所述，我们的结果表明，TM 衍生的 EV 可以介导炎症反应，其蛋白在调节 RAW 264.7 巨噬细胞的功能中发挥关键作用。
Extracellular vesicles (EVs) loaded with proteins, nucleic acids, membrane lipids, and other virulence factors could participate in pathogenic processes in some fungi such as Cryptococcus neoformans and Candida albicans. However, the specific characteristics of EVs derived from Talaromyces marneffei (TM) still have not been figured out yet. In the present study, it has been observed that TM-derived EVs were a heterogeneous group of nanosized membrane vesicles (30–300 nm) under nanoparticle tracking analysis and transmission electron microscopy. The DiI-labeled EVs could be taken up by RAW 264.7 macrophage cells. Incubation of EVs with macrophages would result in increased expression levels of reactive oxygen species, nitric oxide, and some inflammatory factors including interleukin-1β, interleukin-6, interleukin-10, and tumor necrosis factor. Furthermore, the expression of co-stimulatory molecules (CD80, CD86, and MHC-II) was also increased in macrophages stimulated with EVs. The level of inflammatory factors secreted by macrophages showed a significant decrease when EVs were hydrolyzed by protease, while that of DNA and RNA hydrolase treatment remained unchanged. Subsequently, some virulence factors in EVs including heat shock protein, mannoprotein 1, and peroxidase were determined by liquid chromatography–tandem mass spectrometry. Taken together, our results indicated that the TM-derived EVs could mediate inflammatory response and its protein would play a key role in regulating the function of RAW 264.7 macrophage cells.
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DOI：
10.1093/brain/awn101
发表时间：
2008-07
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Pott Godoy MC;Tarelli R;Ferrari CC;Sarchi MI;Pitossi FJ
通讯作者：
Pitossi FJ
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
6.4
作者：
Lau SKP;Lo GCS;Chow FWN;Fan RYY;Cai JJ;Yuen KY;Woo PCY
通讯作者：
Woo PCY
影响因子：
3.8
作者：
Lu, Sha;Li, Dongmei;Calderone, Richard
通讯作者：
Calderone, Richard
影响因子：
14.5
作者：
De Felice, Milena;Ossipov, Michael H.;Porreca, Frank
通讯作者：
Porreca, Frank