A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.
A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.
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DOI：
10.1038/nchembio.1062
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发表时间：
2012-10
影响因子：
14.8
通讯作者：
Wood, Thomas K.
中科院分区：
文献类型：
--
作者：
Wang, Xiaoxue;Lord, Dana M.;Cheng, Hsin-Yao;Osbourne, Devon O.;Hong, Seok Hoon;Sanchez-Torres, Viviana;Quiroga, Cecilia;Zheng, Kevin;Herrmann, Torsten;Peti, Wolfgang;Benedik, Michael J.;Page, Rebecca;Wood, Thomas K.
Among bacterial toxin/antitoxin (TA) systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we demonstrate YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a novel TA system with YjdK (renamed GhoS) since in vitro RNA degradation studies, qRT-PCR, and whole-transcriptome studies revealed GhoS masks GhoT toxicity by cleaving specifically ghoT mRNA. Alanine substitutions showed arginine 28 is important for GhoS activity, and RNA sequencing indicated the GhoS cleavage site is rich in uridine and adenosine. The NMR structure of GhoS indicates it is related to the CAS2 CRISPR RNase, and GhoS is a monomer. Hence, GhoT/GhoS is the first type V TA system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.
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影响因子：
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通讯作者：
FLETCHER, M
影响因子：
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通讯作者：
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