Cytochrome P450 2AA molecular clone, expression pattern, and different regulation by fish oil and lard oil in diets of grass carp (Ctenopharyngodon idella)
Cytochrome P450 2AA molecular clone, expression pattern, and different regulation by fish oil and lard oil in diets of grass carp (Ctenopharyngodon idella)
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草鱼饲料中细胞色素P450 2AA分子克隆、表达模式及鱼油和猪油的不同调节
DOI：
10.1007/s10695-018-0486-0
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发表时间：
2018-05
影响因子：
2.9
通讯作者：
Huang Ji-qin
中科院分区：
文献类型：
--
作者：
Shi Xiao-chen;Sun Jian;Jin Ai;Ji Hong;Yu Hai-bo;Li Yang;Li Xue-xian;Liu Pin;Li Chao;Huang Ji-qin
细胞色素P450酶（CYP酶）催化外源性和内源性底物（包括脂肪酸）的重要代谢反应。本研究首次从草鱼（Ctenopharyngodon idella）中克隆了细胞色素P450 2AA（CYP2AA）基因的完整CDS序列。CYP2AA全长1500 bp，编码499个氨基酸。对C. idella和斑马鱼为86%。它由保守的血红素结合基序FXXGXXXCXG组成。实时荧光定量PCR分析表明，C. Idella在肝脏和脂肪组织中高表达。同时研究了鱼油和猪油对CYP2AA mRNA表达的影响。鱼油（FO）组脂肪组织中CYP2AA的表达显著高于猪油（LO）组（P< 0.01），而肝脏中CYP2AA mRNA的表达无显著差异。提示鱼油可诱导脂肪组织中CYP2AA mRNA的高丰度表达。本研究提供了CYP2AA的分子特征和表达谱，加深了对CYP2AA在鱼类脂质代谢中作用的认识。
Cytochrome P450 enzymes (CYP enzymes) catalyze important metabolic reactions of exogenous and endogenous substrates, including fatty acid. In this study, we cloned the complete CDS of the cytochrome P450 2AA (CYP2AA) gene from the grass carp (Ctenopharyngodon idella) for the first time. CYP2AA consisted of 1500 bp, which encoded a predicted protein of 499 amino acids. The identities of CYP2AA betweenC. idellaand zebrafish were 86%. It consists of the conserved heme-binding motif FXXGXXXCXG. Quantitative real-time PCR analysis indicated that CYP2AA mRNA inC. idellawas highly expressed in liver and adipose tissue. The effects of fish oil and lard oil in diets on expression of CYP2AA mRNA in vivo were also investigated. The fish oil (FO) group exhibited significantly higher CYP2AA expression in adipose tissue than the lard oil (LO) group (P< 0.01), whereas the mRNA expression of CYP2AA was not notably different in liver. It suggested that the high abundance of CYP2AA mRNA expression in adipose tissue could be induced by fish oil. Our findings provided molecular characterization and expression profile of CYP2AA, and enhanced our understanding of CYP2AA in fish lipid metabolism.
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DOI：
10.1098/rstb.2012.0474
发表时间：
2013-02-19
影响因子：
6.3
作者：
Nelson, David R.;Goldstone, Jared V.;Stegeman, John J.
通讯作者：
Stegeman, John J.
DOI：
--
发表时间：
2000-11
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
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DOI：
10.3724/sp.j.1035.2009.50881
发表时间：
2009-09
期刊：
Acta Hydrobiologica Sinica
影响因子：
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作者：
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影响因子：
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日粮非蛋白能源水平对草食性草鱼生长性能、身体成分和脂质代谢的影响（Ctenopharyngodon idella Val.）
影响因子：
2
作者：
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