Signals Required for the Import and Processing of the Alternative Oxidase into Mitochondria*
Signals Required for the Import and Processing of the Alternative Oxidase into Mitochondria*
复制标题
将替代氧化酶导入线粒体并进行加工所需的信号*
DOI：
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发表时间：
1999
影响因子：
4.8
通讯作者：
J. Whelan
中科院分区：
文献类型：
--
作者：
M. Tanudji;S. Sjöling;E. Glaser;J. Whelan
研究了大豆替代氧化酶对植物和动物线粒体的靶向和加工过程中所涉及的关键残留物。进口到大豆线粒体的各种定点突变体表明，整个长度的前序列的阳性残基是重要的进口，而不仅仅是那些在预测的区域的两亲性。阳性残基在前序列C-末端的位置对输入也很重要。用纯化的菠菜线粒体加工肽酶对各种构建体进行的加工试验表明，所有−2位突变体对加工都有显著影响。与进口测定相反，可改变阳性残留物的位置以进行处理。缺失突变体证实了定点诱变数据，因为两亲性α-螺旋不是该同源植物系统中线粒体输入的唯一决定因素。将这些构建体导入大鼠肝线粒体表明抑制程度不同，并且预测的两亲性α-螺旋区域对大鼠肝线粒体更重要。用大鼠肝基质组分处理显示几乎没有抑制作用。这些结果进行了讨论相对于在植物细胞中的靶向特异性，并强调需要进行同源研究，并确定植物线粒体的靶向要求。
The critical residues involved in targeting and processing of the soybean alternative oxidase to plant and animal mitochondria was investigated. Import of various site-directed mutants into soybean mitochondria indicated that positive residues throughout the length of the presequence were important for import, not just those in the predicted region of amphiphilicity. The position of the positive residues in the C-terminal end of the presequence was also important for import. Processing assays of the various constructs with purified spinach mitochondrial processing peptidase showed that all the −2-position mutants had a drastic effect on processing. In contrast to the import assay, the position of the positive residue could be changed for processing. Deletion mutants confirmed the site-directed mutagenesis data in that an amphiphilic α-helix was not the only determinant of mitochondrial import in this homologous plant system. Import of these constructs into rat liver mitochondria indicated that the degree of inhibition differed and that the predicted region of amphiphilic α-helix was more important with rat liver mitochondria. Processing with a rat liver matrix fraction showed little inhibition. These results are discussed with respect to targeting specificity in plant cells and highlight the need to carry out homologous studies and define the targeting requirements to plant mitochondria.
大鼠肝醛脱氢酶的前序列需要在其 N 末端区域存在 α 螺旋，该螺旋通过其 C 末端的螺旋来稳定。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wang,Y;Weiner,H
通讯作者：
Weiner,H
将蛋白质导入线粒体。
DOI：
10.1098/rstb.1988.0036
发表时间：
1988
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Eilers,M;Verner,K;Hwang,S;Schatz,G
通讯作者：
Schatz,G