Serologic cross-reactivity between Class I MHC molecules and an H-2- linked differentiation antigen as detected by monoclonal antibodies
Serologic cross-reactivity between Class I MHC molecules and an H-2- linked differentiation antigen as detected by monoclonal antibodies
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单克隆抗体检测到 I 类 MHC 分子和 H-2 连锁分化抗原之间的血清学交叉反应
DOI：
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发表时间：
1984
影响因子：
15.3
通讯作者：
D. Sachs
中科院分区：
文献类型：
--
作者：
S. Sharrow;L. Flaherty;D. Sachs
抗I类主要组织相容性复合体（MHC）单克隆抗体的免疫荧光和流动显微荧光分析表明一个意想不到的交叉反应。观察到检查的15种抗体中的2种（20-8-4，抗-Kb，Kd，r，s和34-1-2，抗Kd，Dd，Kb，r，s，q，p）可检测到由H-2D右侧基因定位确定的抗原。双色免疫荧光分析表明，这种抗原，不像经典的H-2K和D抗原，在外周T细胞上表达量很高，但只有弱的IA阳性细胞和小的胸腺和骨髓细胞亚群。映射，吸收，阻断和组织分布研究表明，交叉反应性抗原是Qa样的，但不同于以前描述的Qa抗原。因此，这些数据证明了I类MHC抗原和分化抗原之间的血清学交叉反应性，所述抗原由与H-2连锁的基因决定。似乎负责这种新抗原的基因是通过DNA杂交分析检测到的众多I类序列之一，但以前在组织表达方面没有定义。这些数据表明，这些DNA序列中的许多可以在特定组织中表达，并且抗I类单克隆抗体的交叉反应可以为研究这些同源基因的产物提供有用的探针。
Analysis of anti-Class I major histocompatibility complex (MHC) monoclonal antibodies by immunofluorescence and flow microfluorometry demonstrated an unexpected cross-reactivity. Two of fifteen antibodies examined (20-8-4, anti-Kb,Kd,r,s and 34-1-2, antiKd,Dd,Kb,r,s,q,p) were observed to detect an antigen determined by gene(s) mapping to the right of H-2D. Two-color immunofluorescence analysis demonstrated that this antigen, unlike classical H-2K and D antigens, was expressed in high amounts on peripheral T cells, but only weakly on Ia-positive cells and on small subpopulations of thymus and bone marrow cells. Mapping, absorption, blocking, and tissue distribution studies suggested that the cross-reactive antigen is Qa-like, but distinct from previously described Qa antigens. Thus, these data demonstrate serological cross-reactivity between a Class I MHC antigen and a differentiation antigen determined by genes linked to H-2. It seems likely that the gene responsible for this new antigen is one of the numerous Class I-like sequences detected by DNA hybridization analyses, but previously undefined in terms of tissue expression. These data suggest that many of these DNA sequences may be expressed in specific tissues and that cross-reactions of anti-Class I MAbs may provide useful probes for studying the products of such homologous genes.
由 H-2 复合物 K 端控制的第二类 I 抗原的鉴定及其选择性细胞表达。
DOI：
10.1073/pnas.80.5.1445
发表时间：
1983
影响因子：
11.1
作者：
Tryphonas,M;King,DP;Jones,PP
通讯作者：
Jones,PP
CFU-s 上的 Qa-1 和 Qa-2 表达。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lynes,MA;Tonkonogy,S;Flaherty,L
通讯作者：
Flaherty,L
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Cook,RG;Jenkins,RN;Flaherty,L;Rich,RR
通讯作者：
Rich,RR