Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation.
Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation.
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DOI：
10.1111/pce.13872
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Udvardi M
中科院分区：
文献类型：
--
作者：
Ding N;Huertas R;Torres-Jerez I;Liu W;Watson B;Scheible WR;Udvardi M
柳枝稷（Panicum virgatum L.）响应和适应磷（P）限制将有助于优化该物种的P获取和使用，以实现可持续的生物质生产。本综合研究调查了轻度、中度和重度P胁迫对柳枝稷基因组转录和全株代谢、生理和发育的影响。磷限制降低了植物的整体生长，增加了根冠比，在中度磷胁迫下增加了根分枝，在重度磷胁迫下根直径随着根毛密度和长度的增加而减小。RNA测序分析揭示了成千上万的基因，这些基因在中度和重度P胁迫下在根和/或芽中与P-充分植物相比差异表达，其中许多胁迫诱导的基因参与转录和其他形式的调控，初级和次级代谢，运输以及其他参与P-获取和稳态的过程。在后者中有多个miRNA 399基因和这些基因的推定靶点。代谢物分析表明，大多数糖和糖醇的水平随着磷胁迫的增加而下降，而有机酸和氨基酸在轻度和中度磷胁迫下增加，尽管这种趋势在重度磷胁迫下逆转，特别是在地上部。低磷（P）有效性限制了大多数土壤中的植物生长。我们对多年生生物能源作物柳枝稷的磷限制反应进行了多层次分析。阐明柳枝稷科普磷限制的策略和机制将有助于优化磷效率和在低磷条件下最大化生长。
Knowing how switchgrass (Panicum virgatum L.) responds and adapts to phosphorus (P)‐limitation will aid efforts to optimize P acquisition and use in this species for sustainable biomass production. This integrative study investigated the impacts of mild, moderate, and severe P‐stress on genome transcription and whole‐plant metabolism, physiology and development in switchgrass. P‐limitation reduced overall plant growth, increased root/shoot ratio, increased root branching at moderate P‐stress, and decreased root diameter with increased density and length of root hairs at severe P‐stress. RNA‐seq analysis revealed thousands of genes that were differentially expressed under moderate and severe P‐stress in roots and/or shoots compared to P‐replete plants, with many stress‐induced genes involved in transcriptional and other forms of regulation, primary and secondary metabolism, transport, and other processes involved in P‐acquisition and homeostasis. Amongst the latter were multiple miRNA399 genes and putative targets of these. Metabolite profiling showed that levels of most sugars and sugar alcohols decreased with increasing P stress, while organic and amino acids increased under mild and moderate P‐stress in shoots and roots, although this trend reversed under severe P‐stress, especially in shoots. Low phosphorus (P) availability limits plant growth in most soils. We performed a multilevel analysis of P‐limitation responses in the perennial bioenergy crop switchgrass. Elucidation of strategies and mechanisms deployed by switchgrass to cope with P‐limitation will help in efforts to optimize P‐efficiency and maximize growth in low P conditions.
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