Transcriptome analysis of Acidovorax avenae subsp. avenae cultivated in vivo and co-culture with Burkholderia seminalis.
Transcriptome analysis of Acidovorax avenae subsp. avenae cultivated in vivo and co-culture with Burkholderia seminalis.
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燕麦酸食菌亚种的转录组分析。
DOI：
10.1038/srep05698
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发表时间：
2014-07-16
影响因子：
4.6
通讯作者：
Kube M
中科院分区：
文献类型：
--
作者：
Li B;Ibrahim M;Ge M;Cui Z;Sun G;Xu F;Kube M
细菌病原体对环境细菌及其宿主的反应是理解微生物适应性和致病性的关键。在这里，我们使用RNA-Seq全面和定量评估的转录反应的食酸菌燕麦亚种。燕麦菌株RS-1的离体培养、体内培养以及与水稻根际细菌Burkholderia vegetalis R456的共培养。结果显示，对其他细菌有轻微反应，但对宿主有强烈反应。特别是，大量的毒力相关基因编码I型至VI型分泌系统，118个推定的非编码RNA，和7个基因组岛（GI）的差异表达在体内的基础上比较基因组和转录组学分析。此外，11个差异表达的T6 SS基因的敲除突变体的毒力损失强调了这些基因在细菌致病性中的重要性。此外，25个选定的T6 SS基因的定量实时PCR支持通过RNA-Seq获得的表达数据的可靠性。总体而言，这项研究强调了差异表达基因在阐明细菌发病机制中的作用，基于RNA-Seq数据和T6 SS基因敲除的组合分析。
Response of bacterial pathogen to environmental bacteria and its host is critical for understanding of microbial adaption and pathogenesis. Here, we used RNA-Seq to comprehensively and quantitatively assess the transcriptional response of Acidovorax avenae subsp. avenae strain RS-1 cultivated in vitro, in vivo and in co-culture with rice rhizobacterium Burkholderia seminalis R456. Results revealed a slight response to other bacteria, but a strong response to host. In particular, a large number of virulence associated genes encoding Type I to VI secretion systems, 118 putative non-coding RNAs, and 7 genomic islands (GIs) were differentially expressed in vivo based on comparative genomic and transcriptomic analyses. Furthermore, the loss of virulence for knockout mutants of 11 differentially expressed T6SS genes emphasized the importance of these genes in bacterial pathogenicity. In addition, the reliability of expression data obtained by RNA-Seq was supported by quantitative real-time PCR of the 25 selected T6SS genes. Overall, this study highlighted the role of differentially expressed genes in elucidating bacterial pathogenesis based on combined analysis of RNA-Seq data and knockout of T6SS genes.
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影响因子：
30.3
作者：
Jani AJ;Cotter PA
通讯作者：
Cotter PA
影响因子：
4.4
作者：
Khoo JS;Chai SF;Mohamed R;Nathan S;Firdaus-Raih M
通讯作者：
Firdaus-Raih M
影响因子：
1.2
作者：
Li, Bin;Liu, Baoping;Sun, Guochang
通讯作者：
Sun, Guochang
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
影响因子：
4.5
作者：
Cros, Marie-Josee;de Monte, Antoine;Gaspin, Christine
通讯作者：
Gaspin, Christine