Double staining with crystal violet and methylene blue is appropriate for colonic endocytoscopy: an in vivo prospective pilot study.
Double staining with crystal violet and methylene blue is appropriate for colonic endocytoscopy: an in vivo prospective pilot study.
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DOI：
10.1111/den.12164
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Inoue H
中科院分区：
文献类型：
--
作者：
Ichimasa K;Kudo SE;Mori Y;Wakamura K;Ikehara N;Kutsukawa M;Takeda K;Misawa M;Kudo T;Miyachi H;Yamamura F;Ohkoshi S;Hamatani S;Inoue H
细胞内镜检查（EC）在超高放大率下能够在体内可视化胃肠道粘膜的细胞间隙。清晰的图像对于EC的精确诊断至关重要。本研究的目的是评价最佳染色方法EC在结肠。将30例前瞻性入组患者按1：1：1分配至3种不同的染色方法：单独使用0.05%结晶紫（CV）、单独使用1%亚甲蓝（MB）或CV + MB（CM双重）。正常直肠粘膜用每种染料染色并记录EC图像的视频。将染色后细胞核和腺体形成的可见性评价为“可识别”或“不可识别”。测量每个参数变得“可识别”的时间，并比较三种染色方案的平均时间。MB单独和CM双重染色在相当的时间段内产生“可识别的”（102 ± 27 vs 89 ± 22 s，P = 0.263）细胞核，而CV单独不能识别细胞核。CM双染后腺体形成较MB单染快（61 ± 16vs108 ± 24s，P < 0.001）。用CV和MB双重染色，快速提供可识别的细胞核和腺体形成的图像，是结肠EC的适当染色方案。
Endocytoscopy (EC) at ultra-high magnification enables in vivo visualization of cellular atypia of gastrointestinal mucosae. Clear images are essential for precise diagnosis by EC. The aim of the present study was to evaluate the optimal staining method for EC in the colon. Thirty prospectively enrolled patients were allocated 1:1:1 to three distinct staining methods: 0.05% crystal violet (CV) alone, 1% methylene blue (MB) alone, or CV + MB (CM double). Normal rectal mucosae were stained with each dye and videos of EC images were recorded. Visibility of nuclei and gland formation after staining were evaluated as ‘recognizable’ or ‘not recognizable’. Time for each parameter to become ‘recognizable’ was measured, and the average times for the three staining regimens were compared. MB alone and CM double staining resulted in ‘recognizable’ (102 ± 27 vs 89 ± 22 s, P = 0.263) nuclei within comparable periods of time, whereas CV alone was unable to identify nuclei. Gland formation became ‘recognizable’ sooner after CM double staining than after MB alone (61 ± 16 vs 108 ± 24 s, P < 0.001). Double staining with CV and MB, which rapidly provided recognizable images of both nuclei and gland formation, is an appropriate staining regimen for colonic EC.
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影响因子：
9.3
作者：
Eberl, T.;Jechart, G.;Messmann, H.
通讯作者：
Messmann, H.
影响因子：
19.7
作者：
HERMAN, PG;KHAN, A;BODENHEIMER, MM
通讯作者：
BODENHEIMER, MM
影响因子：
7.7
作者：
Canto, MIF;Setrakian, S;Sivak, MV
通讯作者：
Sivak, MV
影响因子：
7.7
作者：
Sasajima, Keita;Kudo, Shin-ei;Shiokawa, Akira
通讯作者：
Shiokawa, Akira
影响因子：
19.7
作者：
Kundel, HL;Polansky, M
通讯作者：
Polansky, M